Anand Ajith, Che Ping, Wu Emily, Jones Todd J
Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA.
Methods Mol Biol. 2019;1931:185-196. doi: 10.1007/978-1-4939-9039-9_13.
Sorghum has been considered a recalcitrant crop for tissue culture and genetic transformation. A breakthrough in Agrobacterium-mediated sorghum transformation was achieved with the use of super-binary cointegrate vectors based on plasmid pSB1. However, even with pSB1, transformation capability was restricted to certain sorghum genotypes, excluding most of the important African sorghum varieties. We recently developed a ternary vector system incorporating the pVIR accessory plasmid. The ternary vector system not only doubled the transformation frequency (TF) in Tx430, but also extended the transformation capability into an important African sorghum elite variety.
高粱一直被认为是一种难以进行组织培养和遗传转化的作物。基于质粒pSB1的超级双元共整合载体的使用，实现了农杆菌介导的高粱转化的突破。然而，即使使用pSB1，转化能力也仅限于某些高粱基因型，排除了大多数重要的非洲高粱品种。我们最近开发了一种包含pVIR辅助质粒的三元载体系统。该三元载体系统不仅使Tx430的转化频率（TF）提高了一倍，还将转化能力扩展到一个重要的非洲高粱优良品种。
