Wu Emily, Zhao Zuo-Yu
DuPont Pioneer, Johnston, IA, USA.
DuPont Pioneer, 501 Hahaione Street, 17K, Honolulu, HI, 96825, USA.
Methods Mol Biol. 2017;1669:355-364. doi: 10.1007/978-1-4939-7286-9_26.
Agrobacterium-mediated plant transformation is commonly used in crop genome modification. An optimized sorghum transformation protocol we developed is described here. Using this protocol, the transformation frequency of sorghum inbred TX430 is over 10% with Agrobacterium strain LBA4404 and 33% with Agrobacterium strain AGL1. Two different selection marker genes, moPAT and PMI, were used in this protocol.
农杆菌介导的植物转化常用于作物基因组修饰。本文描述了我们开发的一种优化的高粱转化方案。使用该方案，高粱自交系TX430用农杆菌菌株LBA4404转化时频率超过10%，用农杆菌菌株AGL1转化时频率为33%。该方案使用了两种不同的选择标记基因，即moPAT和PMI。
