Harris E B, Franzblau S G, Hastings R C
Biochemistry Research Department, GWL Hansen's Disease Center, Carville, Louisiana 70721.
Int J Lepr Other Mycobact Dis. 1988 Dec;56(4):588-91.
The effects of 22 antimicrobial agents on the incorporation of [U14C] palmitic acid ([U14C] PA) into the unique phenolic glycolipid-I (PGL-I) antigen of Mycobacterium leprae were studied. Nude-mouse-propagated M. leprae were incubated in a modified Dubos medium in the presence of antimicrobial agents for 4 days. [U14C] PA was then added and incubation was continued for 8 days. The antileprosy agents dapsone, rifampin, and clofazimine (2 micrograms/ml each) caused a significant reduction in [U14C] PA incorporation into PGL-I. Among other agents, the most active were erythromycin, chloramphenicol, and cerulenin. Low concentrations of ethionamide, tetracycline, and minocycline stimulated label incorporation. This system may prove useful in the evaluation of antileprosy agents.
研究了22种抗菌剂对[U14C]棕榈酸（[U14C]PA）掺入麻风分枝杆菌独特的酚糖脂-I（PGL-I）抗原的影响。将裸鼠繁殖的麻风分枝杆菌在改良的杜博斯培养基中与抗菌剂一起孵育4天。然后加入[U14C]PA并继续孵育8天。抗麻风病药物氨苯砜、利福平和氯法齐明（各2微克/毫升）使[U14C]PA掺入PGL-I的量显著减少。在其他药物中，最有效的是红霉素、氯霉素和浅蓝菌素。低浓度的乙硫异烟胺、四环素和米诺环素刺激标记物掺入。该系统可能在抗麻风病药物的评估中证明有用。
