在BACTEC 460系统中对麻风分枝杆菌进行药物敏感性试验。
Drug susceptibility testing of Mycobacterium leprae in the BACTEC 460 system.
作者信息
Franzblau S G
机构信息
Pharmacology Research Department, Gillis W. Long Hansen's Disease Center, Carville, Louisiana 70721.
出版信息
Antimicrob Agents Chemother. 1989 Dec;33(12):2115-7. doi: 10.1128/AAC.33.12.2115.
Abstract
The susceptibility of Mycobacterium leprae to clinical and experimental antileprosy agents was assessed in the BACTEC 460 system. Nude-mouse-derived M. leprae (10(7) cells), incubated in BACTEC 12B medium at 33 degrees C under reduced oxygen, maintained a fairly constant growth index (14CO2 evolution) for 2 to 3 weeks. At concentrations ranging from 0.031 to 2.0 micrograms/ml, dapsone, rifampin, clofazimine, ethionamide, ofloxacin, clarithromycin, and minocycline all effected reductions in the growth index within 1 to 2 weeks, the extent of inhibition increasing with the incubation time. An in vivo rifampin-resistant isolate displayed markedly reduced susceptibility to rifampin compared with an in vivo-susceptible strain. This system appears to be highly suitable for in vitro drug susceptibility testing of M. leprae.
摘要
在BACTEC 460系统中评估了麻风分枝杆菌对临床和实验性抗麻风病药物的敏感性。源自裸鼠的麻风分枝杆菌（10⁷个细胞），在BACTEC 12B培养基中于33℃、低氧条件下培养，在2至3周内保持相当恒定的生长指数（¹⁴CO₂释放）。在浓度范围为0.031至2.0微克/毫升时，氨苯砜、利福平、氯法齐明、乙硫异烟胺、氧氟沙星、克拉霉素和米诺环素在1至2周内均使生长指数降低，抑制程度随培养时间增加。与体内敏感菌株相比，一株体内耐利福平分离株对利福平的敏感性明显降低。该系统似乎非常适合用于麻风分枝杆菌的体外药物敏感性测试。
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