Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi 39253, Korea.
Sensors (Basel). 2019 Jan 16;19(2):357. doi: 10.3390/s19020357.
In this paper, we proposed cancer cell acoustic stimulation by shunt-diode pre-linearizer scheme using a very high frequency (≥100 MHz) piezoelectric transducer. To verify the concept of our proposed scheme, we performed pulse-echo detection, and accessed therapeutic effects of human cervical cancer cells exposed to acoustic stimulation experiments using 100 MHz focused piezoelectric transducer triggered by PA with and without the proposed shunt-diode pre-linearizer scheme. In the pulse-echo detection responses, the peak-to-peak voltage of the echo signal when using the PA with shunt-diode pre-linearizer (49.79 mV) was higher than that when using the PA alone (29.87 mV). In the experimental results, the cell densities of cancer cells on Day 4 when using no acoustic stimulation (control group), the very high-frequency piezoelectric transducer triggered by PA only and PA combined with proposed pre-linearizer schemes (1 V and 5 V DC bias voltages) showed 100%, 92.8 ± 4.2%, 84.2 ± 4.6%, and 78 ± 2.9%, respectively. Therefore, we confirmed that the shunt-diode pre-linearizer could improve the performances of the pulse signals of the PA, thus, enabling better therapeutic stimulation performances for cancer cell suppression.
在本文中，我们提出了一种通过使用非常高频率（≥100MHz）的压电换能器的分流二极管预线性化器方案对癌细胞进行声刺激。为了验证我们提出的方案的概念，我们进行了脉冲回波检测，并使用 100MHz 聚焦压电换能器通过 PA 进行了声刺激实验，评估了人宫颈癌细胞的治疗效果，PA 分别带有和不带有我们提出的分流二极管预线性化器方案。在脉冲回波检测响应中，使用带分流二极管预线性化器的 PA（49.79mV）的回波信号的峰峰值电压高于仅使用 PA（29.87mV）的情况。在实验结果中，在第 4 天没有声刺激（对照组）、仅由 PA 触发的非常高频率压电换能器和由 PA 与提出的预线性化器方案（1V 和 5V 直流偏置电压）结合触发的非常高频率压电换能器时，癌细胞的细胞密度分别为 100%、92.8±4.2%、84.2±4.6%和 78±2.9%。因此，我们证实了分流二极管预线性化器可以改善 PA 脉冲信号的性能，从而实现更好的癌症细胞抑制治疗刺激性能。
