Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Universitat Rovira i Virgili, Tarragona, 43007, Spain.
Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Centro di ricerca per gli Alimenti e la Nutrizione, Roma, 00178, Italy.
Mol Nutr Food Res. 2019 Apr;63(8):e1800720. doi: 10.1002/mnfr.201800720. Epub 2019 Jan 23.
Intestinal dysfunction consists of a defective barrier function, which allows the influx of luminal endotoxins, thus causing intestinal inflammation. Proanthocyanidins are natural bioactive compounds that could modulate intestinal dysfunction. This study analyzes the protective effects of proanthocyanidins in a rat model of intestinal dysfunction.
To investigate the preventive effects of both high dietary (75 mg kg body weight) and pharmacological (375 mg kg body weight) oral doses of proanthocyanidins (GSPE), rat intestinal dysfunction is induced with LPS (i.p.). In vivo intestinal permeability (ovalbumin [OVA] assay) and systemic inflammation and endotoxemia (TNF-α and LPS plasma levels) are assessed. Intestinal inflammation and oxidative stress are determined using myeloperoxidase (MPO), cyclooxygenase-2 (COX-2) activities, and reactive oxygen species (ROS) levels, respectively. Ileal gene expression of permeability/inflammatory genes is analyzed. LPS administration induces intestinal permeability, inflammation, and oxidative stress. GSPE normalizes in vivo OVA levels. In the small intestine, the GSPE treatment decreases MPO and COX-2 activities; modulates the ileum inflammatory and permeability proteins gene expression; and in the large intestine, prevents increase of ROS levels.
Proanthocyanidins, at nutritional and pharmacological doses, prevents endotoxin-induced-intestinal inflammation, permeability, and oxidative stress in rats differentially in each intestinal section. Proanthocyanidins are nutritional-therapeutic novel candidates for preventing intestinal dysfunction.
肠道功能障碍包括屏障功能缺陷，这允许腔内内毒素流入，从而导致肠道炎症。原花青素是一种天然生物活性化合物，可以调节肠道功能障碍。本研究分析了原花青素在大鼠肠道功能障碍模型中的保护作用。
为了研究高膳食（75mg/kg 体重）和药理学（375mg/kg 体重）剂量的原花青素（GSPE）的预防作用，用 LPS（腹腔内）诱导大鼠肠道功能障碍。体内肠道通透性（卵清蛋白[OVA]测定）和全身炎症和内毒素血症（TNF-α 和 LPS 血浆水平）进行评估。用髓过氧化物酶（MPO）、环氧化酶-2（COX-2）活性和活性氧（ROS）水平分别测定肠道炎症和氧化应激。分析回肠通透性/炎症基因的表达。LPS 给药诱导肠道通透性、炎症和氧化应激。GSPE 使体内 OVA 水平正常化。在小肠中，GSPE 处理降低 MPO 和 COX-2 活性；调节回肠炎症和通透性蛋白基因表达；并在大肠中，防止 ROS 水平升高。
原花青素以营养和药理剂量给药，可预防内毒素诱导的大鼠各肠段的肠道炎症、通透性和氧化应激。原花青素是预防肠道功能障碍的新型营养治疗候选药物。
