MoBioFood Research Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Marcel·lí Domingo 1. PC, 43007, Tarragona. Spain.
MoBioFood Research Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Marcel·lí Domingo 1. PC, 43007, Tarragona. Spain.
J Nutr Biochem. 2018 Dec;62:35-42. doi: 10.1016/j.jnutbio.2018.07.012. Epub 2018 Aug 30.
The consumption of Westernized diets leads to hyperphagia and obesity, as well as intestinal alterations. In the present study, we evaluated the effect of the administration of a grape seed proanthocyanidin extract (GSPE) at different time points on the modulation of intestinal barrier function (intestinal permeability and metabolic endotoxemia), in rats with high-fat/high-carbohydrate diet-induced obesity. Animals were fed a cafeteria diet (CAF) supplemented with a preventive (PRE-CAF) or simultaneously intermittent (SIT-CAF) GSPE treatment (500 mg/kg bw). Changes in the plasma levels of an orally administered marker of intestinal permeability (ovalbumin, OVA), lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α) were analyzed after animals were fed the obesogenic diet for 8, 12 and 17 weeks. In addition, ex vivo variations in transepithelial electrical resistance (TEER), the expression of tight junction (TJ) genes and the activity of myeloperoxidase (MPO) in the small and large intestines were monitored at the end of the experiment. The CAF diet increased OVA, LPS, MPO and TNF-α levels, accompanied by decreased TEER values in the small and large intestines. Interestingly, both GSPE treatments prevented these detrimental effects of the CAF diet, being the SIT-CAF group the most effective after 17 weeks of diet intervention. For the first time, this study provides evidence of the ameliorative effect of a proanthocyanidin extract, administered before or together with an obesogenic diet, on barrier dysfunction, as measured by intestinal permeability and metabolic endotoxemia.
西式饮食的消费会导致过食和肥胖，以及肠道改变。在本研究中，我们评估了在不同时间点给予葡萄籽原花青素提取物 (GSPE) 对高脂肪/高碳水化合物饮食诱导肥胖大鼠肠道屏障功能（肠道通透性和代谢内毒素血症）调节的影响。动物喂食自助饮食（CAF），并补充预防性（PRE-CAF）或同时间歇性（SIT-CAF）GSPE 治疗（500mg/kg bw）。在动物喂食致肥胖饮食 8、12 和 17 周后，分析口服肠道通透性标志物（卵白蛋白，OVA）、脂多糖（LPS）和肿瘤坏死因子-α（TNF-α）的血浆水平变化。此外，在实验结束时监测了小肠和大肠上皮细胞间紧密连接（TJ）基因表达和髓过氧化物酶（MPO）活性的跨上皮电阻（TEER）变化。CAF 饮食增加了 OVA、LPS、MPO 和 TNF-α水平，同时降低了小肠和大肠的 TEER 值。有趣的是，两种 GSPE 处理均预防了 CAF 饮食的这些不利影响，SIT-CAF 组在饮食干预 17 周后效果最明显。这项研究首次提供了证据，证明原花青素提取物在给予致肥胖饮食之前或同时给予，可改善屏障功能障碍，如肠道通透性和代谢内毒素血症所测。
