Shi Huiyu, Yang Fengyuan, Peng Zeru, Wu Junlong, Wang Qin, Qiu Pingfei, Bao Ruiying, Huang Liangmin, Li Xiaochun, Zhang Haiwen, Wang Xuemei
Department of Animal Science and Technology, School of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
Front Vet Sci. 2024 Nov 18;11:1369515. doi: 10.3389/fvets.2024.1369515. eCollection 2024.
Lipopolysaccharides (LPS) are commonly used to construct inflammation models. However, poultry have a certain degree of tolerance to LPS due to the lack of thrombin XI and XII in their bodies. Thrombin activation produces clotting factors that can cleave prothrombin to form thrombin. The purpose of this study was to construct a chick oxidative stress model used different concentrations of LPS combined with thrombin in order to screen for the optimal concentration for constructing the oxidative stress model, and to explore the effects of this stimulus on various indicators of chicks. Eighty-one young chicks (4-days-old) were randomly divided into three groups with 27 chicks per group where each group contained 3 replicates with 9 birds each: a control group (physiological saline), a low-dose group (LPS 5 mg/kg thrombin 150 U/kg), and a high-dose group (LPS 10 mg/kg thrombin 300 U/kg). The results indicated that compared with the control group, the low-dose group and the high-dose group significantly increased the content of Malondialdehyde (MDA) in serum and reduced the content of T-AOC, GSH-PX and SOD, respectively. Meanwhile, the levels of NO and inflammatory factors IL-1β, IL-6 and TNF-α TNF-α in the liver were significantly increased in the low-dose and high-dose groups compared with the control group, respectively. Liver and thymus tissue sections from the low- and high-dose groups showed hemorrhage, hemolysis, and a small amount of exudation. In terms of inflammatory effect, the serum MDA content and the levels of NO, IL-1β, IL-6 and TNF-α factors in the liver were significantly increased in the low-dose group compared with the high-dose group. On histopathological observation, tissue damage was more pronounced in the low-dose group than in the high-dose group. In conclusion, LPS combined with thrombin could induce oxidative stress in chicks and the pathological changes of the low-dose effect are more pronounced.
脂多糖（LPS）常用于构建炎症模型。然而，家禽由于体内缺乏凝血因子XI和XII，对LPS具有一定程度的耐受性。凝血酶激活会产生凝血因子，这些凝血因子可裂解凝血酶原形成凝血酶。本研究的目的是构建雏鸡氧化应激模型，采用不同浓度的LPS与凝血酶联合使用，以筛选构建氧化应激模型的最佳浓度，并探讨这种刺激对雏鸡各项指标的影响。81只4日龄雏鸡随机分为三组，每组27只，每组包含3个重复，每个重复9只：对照组（生理盐水）、低剂量组（LPS 5mg/kg + 凝血酶150U/kg）和高剂量组（LPS 10mg/kg + 凝血酶300U/kg）。结果表明，与对照组相比，低剂量组和高剂量组血清中丙二醛（MDA）含量显著增加，而总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）含量分别降低。同时，与对照组相比，低剂量组和高剂量组肝脏中一氧化氮（NO）和炎症因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平显著升高。低剂量组和高剂量组的肝脏和胸腺组织切片显示出血、溶血和少量渗出。在炎症效应方面，低剂量组血清MDA含量以及肝脏中NO、IL-1β、IL-6和TNF-α因子水平与高剂量组相比显著升高。在组织病理学观察中，低剂量组的组织损伤比高剂量组更明显。综上所述，LPS联合凝血酶可诱导雏鸡氧化应激，且低剂量效应的病理变化更明显。
