Department of General Surgery, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China.
Graduate School, The First Clinical School of Nanjing Medical University, Nanjing 210029, China.
Epigenomics. 2019 Feb;11(2):199-213. doi: 10.2217/epi-2017-0167. Epub 2019 Jan 18.
The study aimed to investigate the role of circular RNA circASS1 in breast cancer cells.
MATERIALS & METHODS: Circular RNAs microarray expression profile were analyzed in MCF-7, MDA-MB-231, and qRT-PCR and western blotting were used to quantify expression of circASS1 and its parental gene ASS1. Wound healing, migration and invasion assay were performed. Luciferase assay system was used to detect harbored miRNA.
CircASS1 in MDA-MB-231 is downregulated comparing to MCF-7, and overexpression of circASS1 could suppress invasion and migration. While silence, it could promote invasion and migration. MiR-4443 functioning as a tumor promoter gene could be captured by circASS1. ASS1 is upregulated in loss-of-function experiments, while downregulated in gain-of-function experiments.
CircASS1 suppresses invasion and migration capacity of breast cancer cells and harbored miR-4443.
本研究旨在探讨环状 RNA circASS1 在乳腺癌细胞中的作用。
分析 MCF-7、MDA-MB-231 中的环状 RNA 微阵列表达谱，并采用 qRT-PCR 和 Western blot 定量检测 circASS1 及其亲本基因 ASS1 的表达。进行划痕愈合、迁移和侵袭实验。利用荧光素酶报告基因检测系统检测其潜在的 miRNA。
与 MCF-7 相比，MDA-MB-231 中的 circASS1 下调，过表达 circASS1 可抑制侵袭和迁移。沉默时，它可以促进侵袭和迁移。miR-4443 作为一种肿瘤促进基因可被 circASS1 捕获。ASS1 在功能丧失实验中上调，而在功能获得实验中下调。
circASS1 抑制乳腺癌细胞的侵袭和迁移能力，并可作为 miR-4443 的宿主。
