Recherches Translationelles sur VIH et Maladies Infectieuses/Institut national de la santé et de la recherche médicale, Institut de Recherche pour le Développement and University of Montpellier, France.
Institut National de Recherche Biomédicales, Kinshasa, Democratic Republic of the Congo (DRC).
J Infect Dis. 2019 Oct 8;220(10):1599-1608. doi: 10.1093/infdis/jiz006.
Bats are considered a reservoir species for Ebola viruses, but nonhuman primates (NHPs) have represented a source of infection in several outbreaks in humans. Here we report serological screening of blood or fecal samples from monkeys (n = 2322) and apes (n = 2327). Thirty-six NHP species from Cameroon, Democratic Republic of the Congo, and Ivory Coast were tested with a sensitive and specific Luminex-based assay for immunoglobulin G antibodies to 4 Ebola virus species. Using the simultaneous presence of antibodies to nucleoproteins and glycoproteins to define positivity, we showed that specific Ebola virus antibodies are not widespread among NHPs. Only 1 mustached monkey (Cercopithecus cephus) from Cameroon was positive for Sudan ebolavirus. These observations support that NHPs are most likely intermediate hosts for Ebola viruses. With the increasing frequency of Ebola outbreaks, it is crucial to identify the animal reservoir and understand the ecology of Ebola viruses to inform disease control.
蝙蝠被认为是埃博拉病毒的储存宿主，但在人类的几次疫情中，非人类灵长类动物（NHPs）是感染源。在此，我们报告了对来自喀麦隆、刚果民主共和国和科特迪瓦的 2322 只猴子和 2327 只猿的血液或粪便样本进行的血清学筛查。我们使用一种基于 Luminex 的灵敏且特异的免疫球蛋白 G 抗体检测方法，对 4 种埃博拉病毒种属检测了 36 种灵长类动物。使用核蛋白和糖蛋白抗体同时存在来定义阳性，我们表明埃博拉病毒特异性抗体在 NHPs 中并不广泛存在。只有一只来自喀麦隆的须长尾猴（Cercopithecus cephus）对苏丹埃博拉病毒呈阳性。这些观察结果支持 NHPs 最有可能是埃博拉病毒的中间宿主。随着埃博拉疫情的频发，确定动物储存宿主并了解埃博拉病毒的生态学对于指导疾病控制至关重要。
