Huang Lixing, Zuo Yanfei, Jiang Qingling, Su Yongquan, Qin Yingxue, Xu Xiaojin, Zhao Lingmin, Yan Qingpi
Key Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Ministry of Agriculture, Jimei University, Xiamen, China.
State Key Laboratory of Large Yellow Croaker Breeding, Ningde, China.
J Fish Dis. 2019 Mar;42(3):431-446. doi: 10.1111/jfd.12957. Epub 2019 Jan 18.
Pseudomonas plecoglossicida is associated with multiple fish diseases, and temperature is one of the most important environmental factors related to its outbreak. To elucidate the influence of temperature variation on the pathogen, the global metabolomics of P. plecoglossicida (NZBD9) were analysed at the virulent (18°C) and avirulent (12°C and 28°C) temperatures. The result showed that the levels of Phosphoric acid, Tyrosine, Spermidine and Sucrose were significantly reduced，while Itaconic acid, Glucaric acid and Isomaltose were increased in P. plecoglossicida at 18°C. These metabolic adjustments assist P. plecoglossicida to survive in adverse environments, proliferate in the host, colonize and resist host immune clearance during the initial steps of infection. The results suggested that L321_03626 and L321_18122 genes played a key role in the regulation of these metabolic adaptions and thus regulated P. plecoglossicida virulence at virulent temperature, which was proved by further gene silencing and artificial infection. The present study, for the first time, determines the P. plecoglossicida metabolomic responses to temperature variation, which is helpful to explore its pathogenic mechanism and provides reference for disease control.
嗜水气单胞菌与多种鱼类疾病相关，温度是与其爆发相关的最重要环境因素之一。为阐明温度变化对该病原菌的影响，对嗜水气单胞菌（NZBD9）在致病温度（18°C）和非致病温度（12°C和28°C）下进行了全代谢组学分析。结果表明，在18°C时，嗜水气单胞菌中磷酸、酪氨酸、亚精胺和蔗糖的水平显著降低，而衣康酸、葡糖二酸和异麦芽糖的水平升高。这些代谢调节有助于嗜水气单胞菌在不利环境中生存，在宿主体内增殖，在感染初期定殖并抵抗宿主免疫清除。结果表明，L321_03626和L321_18122基因在这些代谢适应的调节中起关键作用，从而在致病温度下调节嗜水气单胞菌的毒力，这通过进一步的基因沉默和人工感染得到证实。本研究首次确定了嗜水气单胞菌对温度变化的代谢组学反应，有助于探索其致病机制，并为疾病控制提供参考。
