Department of Basic Health Sciences, Laboratory of Cellular and Molecular Immunology, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil.
Department of Basic Health Sciences, Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil.
J Cell Biochem. 2019 Jun;120(6):10726-10736. doi: 10.1002/jcb.28364. Epub 2019 Jan 20.
To investigate the impact of physical fitness on the mobilization of CD4 CD25 CD39 and CD4 CD25 CD39 T cells in response to acute exercise.
Fifteen high physical fitness (25.3 ± 1.4 years) and 15 low physical fitness (26.1 ± 1.9 years) men performed a single bout of high-intensity interval exercise (HIIE, 10 bouts of 60 seconds at 85% HRmax intercepted by 75 seconds of recovery at 50% HRmax). Blood lymphocytes were isolated before, immediately after and 1 hour after exercise for assessment of cell surface expression of CD25, CD39, and CD73 on CD4 T cells. Effector memory T cells (mTeff) were identified by CD4 CD25 CD39 coexpression, and memory regulatory T cells (mTReg) were defined as CD4 CD25 CD39 T cells.
Exercise increased CD4 and CD4 CD25 T cell frequencies immediately after followed by a decrease bellow to baseline values at 1 hour after the bout in both low and high physical fitness groups. At baseline, the proportions of mTeff were higher, while mTreg were lower in low physical fitness individuals. The frequency of mTreg increased immediately after HIIE in both groups, and remained higher 1 hour after the bout. However, high physical fitness individuals presented higher mTreg frequency in all periods evaluated. A significantly mobilization of mTeff cells was identified in both groups immediately after HIIE. High physical fitness individuals displayed a decrease in mTeff cells bellow to baseline, while the frequency of mTeff remained higher in low physical fitness group 1 hour after the bout. The peripheral frequency of CD4 CD25 CD73 T cells increased in a similar way immediately after the bout in both groups, returning to the baseline values 1 hour after exercise. No differences in CD4 CD25 CD73 T cells were observed after HIIE in both groups.
Our results highlight the impact of physical activity status in the redistribution of CD4 T cells expressing ectonucleotidases in response to HIIE.
探讨体能对急性运动后 CD4 CD25 CD39 和 CD4 CD25 CD39 T 细胞动员的影响。
15 名高体能（25.3±1.4 岁）和 15 名低体能（26.1±1.9 岁）男性进行单次高强度间歇运动（HIIE，10 个 60 秒 85%最大心率冲刺，75 秒 50%最大心率恢复）。运动前、运动后即刻和 1 小时后分离血淋巴细胞，评估 CD4 T 细胞表面 CD25、CD39 和 CD73 的表达。通过 CD4 CD25 CD39 共表达鉴定效应记忆 T 细胞（mTeff），并将 CD4 CD25 CD39 T 细胞定义为记忆调节性 T 细胞（mTReg）。
运动后即刻，两组的 CD4 和 CD4 CD25 T 细胞频率均增加，随后在运动后 1 小时降至基线以下。在基线时，低体能个体的 mTeff 比例较高，而 mTreg 比例较低。HIIE 后即刻两组 mTreg 频率均增加，运动后 1 小时仍保持较高水平。然而，高体能个体在所有评估时间段均显示出较高的 mTreg 频率。HIIE 后即刻两组均鉴定出 mTeff 细胞的显著动员。高体能个体的 mTeff 细胞数量减少至低于基线，而低体能个体的 mTeff 频率在运动后 1 小时仍保持较高水平。HIIE 后即刻两组外周血 CD4 CD25 CD73 T 细胞频率均以相似方式增加，运动后 1 小时恢复至基线值。两组 HIIE 后 CD4 CD25 CD73 T 细胞均无差异。
我们的结果强调了体能活动状态对 HIIE 后 CD4 T 细胞表达胞外核苷酸酶再分布的影响。
