Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea.
Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea; Human and Environmental Toxicology Program, Korea University of Science and Technology (UST), Daejeon, Republic of Korea.
J Hazard Mater. 2019 Apr 15;368:97-103. doi: 10.1016/j.jhazmat.2019.01.034. Epub 2019 Jan 14.
Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) have widely and frequently used in many industrial sectors, and thus have been frequently found in the environment. These chemicals may act as endocrine disrupting chemicals (EDCs), although the molecular mechanisms are still debated. In this study, Japanese medaka (Oryzias latipes) were exposed to 10 mg/l PFOA and 1 mg/l PFOS for 21 days, and the reproductive responses, such as the fecundity, secondary sexual characteristics and transcriptional levels of vitellogenin (vtg1 and vtg2) and choriogenin (chgh, chghm and chgl), were time-dependently evaluated (day 7, 14 and 21). PFOA and PFOS significantly reduced fecundity, and caused expression changes in the genes with time, although the patterns were different for each chemical and each sex. Different transcriptional regulations of vitellogenin and choriogenin in male suggest that PFOA and PFOS have different mode of actions in reproductive effects despite their similar chemical structure.
全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）广泛应用于许多工业领域，因此在环境中经常被发现。这些化学物质可能具有内分泌干扰物（EDCs）的作用，尽管其分子机制仍存在争议。在这项研究中，日本青鳉（Oryzias latipes）暴露于 10mg/L 的 PFOA 和 1mg/L 的 PFOS 中 21 天，其繁殖反应，如生殖力、第二性征和卵黄蛋白原（vtg1 和 vtg2）和壳质蛋白原（chgh、chghm 和 chgl）的转录水平，随时间呈现出依赖性变化（第 7、14 和 21 天）。PFOA 和 PFOS 显著降低了生殖力，并随时间导致基因表达发生变化，尽管每种化学物质和性别之间的模式都不同。雄性卵黄蛋白原和壳质蛋白原的不同转录调控表明，尽管它们具有相似的化学结构，但 PFOA 和 PFOS 在生殖效应中具有不同的作用模式。
