Lassoued K, Guilly M N, Andre C, Paintrand M, Dhumeaux D, Danon F, Brouet J C, Courvalin J C
Laboratory of Immunochemistry and Immunopathology, INSERM U 108, Paris, France.
Clin Exp Immunol. 1988 Nov;74(2):283-8.
Fifty sera which gave ring-like nuclear staining in immunofluorescence on rat liver tissue sections were characterized. Using immunoprecipitation and Western blotting we showed that 10 of the sera contained antibodies to 200 kD polypeptide(s) of nuclear envelope. Clinical and biological data were available for nine of the patients. Strikingly, all of these patients suffered from primary biliary cirrhosis with eight of them having anti-mitochondrial antibodies. As no control serum displayed such a reactivity, anti-200 kD polypeptide(s) antibodies can be considered as a new marker specific of a subset of primary biliary cirrhosis, being present even when anti-mitochondrial antibodies are absent. The exact identity of the target remains to be established, since several polypeptides of similar molecular weight have been reported to belong to the nuclear envelope.
对在大鼠肝组织切片免疫荧光中呈现环状核染色的50份血清进行了特征分析。通过免疫沉淀和蛋白质印迹法，我们发现其中10份血清含有针对核膜200kD多肽的抗体。9名患者有临床和生物学数据。令人惊讶的是，所有这些患者都患有原发性胆汁性肝硬化，其中8人有抗线粒体抗体。由于没有对照血清显示出这种反应性，抗200kD多肽抗体可被视为原发性胆汁性肝硬化一个亚组的新特异性标志物，即使在没有抗线粒体抗体时也存在。由于据报道几种分子量相似的多肽属于核膜，因此目标的确切身份仍有待确定。
