Department of Prosthodontics, Propaedeutics and Dental Materials, School of Dentistry, Christian-Albrechts University Kiel, Arnold-Heller Str. 16, 24105, Kiel, Germany.
Institute of Immunology, Christian-Albrechts University Kiel, Kiel, Germany.
Clin Oral Investig. 2019 Oct;23(10):3731-3738. doi: 10.1007/s00784-019-02801-7. Epub 2019 Jan 21.
The aim of this in vitro study was to assess changes in the gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces conditioned by UV light. To this end, expression levels of proinflammatory cytokines were analyzed in vitro in human whole blood.
Dental implants made of grade 4 titanium were conditioned by UV light in a UV device and submerged in human whole blood. Unconditioned implants served as controls, and blood samples without implants served as the negative control group. Sampling was performed at 1, 8, and 24 h. Changes in the expression levels of interleukin-1β (IL1B) and tumor necrosis factor alpha (TNF) were assessed using RT-qPCR at the mRNA level.
The gene expression of IL1B was significantly suppressed in the test group over the observation period compared to the control group during the 1-8 h after having contact between the implant surface and the blood. The gene expression of TNF was not significantly altered by UV conditioning after 1 and 8 h of observation, but both cytokine expression levels were increased significantly after 24 h.
Differences in the gene expression of proinflammatory cytokines after insertion of UV-conditioned titanium implants can be assessed using a human whole blood test. UV-conditioned implant surfaces apparently suppress the release of IL1B in vitro.
The results of our publication demonstrate that modulation of the early inflammatory response in human whole blood is possible by surface treatment with UV light. In particular, the suppression of IL1B expression, especially after the initial contact of blood cells, may be beneficial in the osseointegration of titanium implants by positively influence the balance between rejection and acceptance of an implant.
本体外研究旨在评估经紫外线（UV）光条件处理的钛种植体表面与人体全血接触后促炎细胞因子基因表达的变化。为此，我们在体外分析了人类全血中促炎细胞因子的表达水平。
用 UV 装置对 4 级钛制成的牙种植体进行 UV 条件处理，并将其浸入人体全血中。未条件处理的种植体作为对照，无种植体的血样作为阴性对照组。在 1、8 和 24 小时时进行采样。使用 RT-qPCR 在 mRNA 水平上评估白细胞介素 1β (IL1B) 和肿瘤坏死因子 α (TNF) 的表达水平变化。
与对照组相比，在接触种植体表面和血液后的 1-8 小时内，实验组的 IL1B 基因表达在整个观察期间均受到显著抑制。在观察的 1 和 8 小时后，TNF 的基因表达未因 UV 条件处理而发生显著改变，但两种细胞因子的表达水平在 24 小时后均显著增加。
使用人体全血检测可以评估插入经 UV 条件处理的钛种植体后促炎细胞因子的基因表达差异。UV 条件处理的种植体表面显然在体外抑制了 IL1B 的释放。
我们的研究结果表明，通过表面 UV 处理可以调节人类全血中的早期炎症反应。特别是，在血细胞初始接触后抑制 IL1B 的表达可能有益于钛种植体的骨整合，通过积极影响对植入物的排斥和接受之间的平衡来实现。
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