Department of Prosthodontics, Propaedeutics and Dental Materials, Christian-Albrechts University of Kiel, Germany.
Institute of Immunology, Christian-Albrechts University of Kiel, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts University of Kiel, Germany.
J Craniomaxillofac Surg. 2019 Aug;47(8):1255-1261. doi: 10.1016/j.jcms.2019.05.004. Epub 2019 May 9.
The aim of this in vitro study was to identify changes in gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces after plasma treatment.
Grade 4 titanium dental implants were conditioned with low-pressure plasma (LPP) and atmospheric-pressure plasma (APP) and submerged in human whole blood in vitro. Unconditioned implants and blood samples without implants served as control and negative control groups, respectively. Sampling was performed at 1, 8, and 24 h. Changes in mRNA expression levels of interleukin 1-beta (IL1-β) and tumor necrosis factor-alpha (TNF-α) were assessed using RT-qPCR.
In the control group, significant increases in IL1-β and TNF-α expression were observed. Significant decreases in the expression of IL1-β and TNF-α were identified in blood with implants after plasma treatment.
Differences in gene expression of proinflammatory cytokines after incubation of plasma-conditioned titanium implants can be assessed using human whole blood. The results of the present study indicate that plasma treatment (APP and LPP) of titanium dental implants leads to downregulation of proinflammatory cytokine gene expression, which might be beneficial in early osseointegration.
本体外研究的目的是鉴定经过等离子体处理后的钛种植体表面与人类全血接触后促炎细胞因子基因表达的变化。
四级钛牙种植体用低压等离子体（LPP）和常压等离子体（APP）预处理，并在体外浸入人类全血中。未处理的种植体和无种植体的血液样本分别作为对照和阴性对照。分别在 1、8 和 24 小时进行取样。使用 RT-qPCR 评估白细胞介素 1-β（IL1-β）和肿瘤坏死因子-α（TNF-α）mRNA 表达水平的变化。
在对照组中，观察到 IL1-β 和 TNF-α 的表达显著增加。经过等离子体处理的含种植体血液中 IL1-β 和 TNF-α 的表达显著降低。
使用人类全血可以评估等离子体处理的钛种植体孵育后促炎细胞因子基因表达的差异。本研究结果表明，钛牙种植体的等离子体处理（APP 和 LPP）导致促炎细胞因子基因表达下调，这可能有益于早期骨整合。
