Zhao Fei, Chen Chu-Wen, Yang Wen-Wen, Xu Li-Hua, Du Zhi-Hao, Ge Xi-Yuan, Li Sheng-Lin
Central Laboratory, Peking University School and Hospital of Stomatology& National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology &Beijing Key Laboratory of Digital Stomatology, Beijing, 10081, China.
Cell Mol Biol (Noisy-le-grand). 2018 Dec 31;64(15):100-106.
Circular RNAs(circRNA) are recently demonstrated to have a close relationship with tumors.To investigate the role of circular RNA in the pathogenesis of salivary adenoid cystic carcinoma(SACC), ten SACC tissues and paired normal submandibular gland(SMG) tissues were collected as the tumor group and the control group. Total RNA was extracted and then measured using ceRNA microarray (including mRNA, lncRNA, and circRNA) and miRNA microarray. Gene Ontology(GO) analysis and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway analysis were performed in order to investigate the function of the differential expressing genes. The ceRNA regulatory network was constructed to find the core circRNAs. Then the role of circRNA on proliferation was examined in the SACC cell line SACC-83 using CCK-8,qRT-PCR and western blotting, and its roles on migration and invasion were examined using wound healing assay and transwell assay. The results of the microarrays showed that 3792 mRNAs, 7649 lncRNAs, 11553 circRNAs, and 132 miRNAs expressed differentially. The ceRNA regulatory network analysis showed that hsa_circ_0059655 and other 14circRNAs derived from PYGB target on several similar genes by miR-338-3p.Among the 15 circRNAs derived from PYGB, hsa_circ_0059655has the most relationships in the ceRNA network. Furthermore, after hsa_circ_0059655 was knocked down in SACC-83 cells, the expression of hsa-miR-338-3p was up-regulated while CCND1was down-regulated. The proliferation, migration, and invasion of SACC-83 cells also decreased after hsa_circ_0059655 knock-downed.Taken together, the circRNAs derived from PYGB may regulate the tumorigenesis and development of SACC through competing with miR-338-3p.
环状RNA（circRNA）最近被证明与肿瘤密切相关。为了研究环状RNA在涎腺腺样囊性癌（SACC）发病机制中的作用，收集了10例SACC组织及配对的正常下颌下腺（SMG）组织作为肿瘤组和对照组。提取总RNA，然后使用ceRNA微阵列（包括mRNA、lncRNA和circRNA）和miRNA微阵列进行检测。进行基因本体论（GO）分析和京都基因与基因组百科全书（KEGG）通路分析以研究差异表达基因的功能。构建ceRNA调控网络以寻找核心circRNA。然后使用CCK-8、qRT-PCR和蛋白质印迹法检测circRNA在SACC细胞系SACC-83中对增殖的作用，并使用伤口愈合试验和Transwell试验检测其对迁移和侵袭的作用。微阵列结果显示，有3792个mRNA、7649个lncRNA、11553个circRNA和132个miRNA差异表达。ceRNA调控网络分析表明，来自PYGB的hsa_circ_0059655和其他14个circRNA通过miR-338-3p靶向几个相似基因。在来自PYGB的15个circRNA中，hsa_circ_0059655在ceRNA网络中的关系最多。此外，在SACC-83细胞中敲低hsa_circ_0059655后，hsa-miR-338-3p的表达上调，而CCND1的表达下调。敲低hsa_circ_0059655后，SACC-83细胞的增殖、迁移和侵袭也减少。综上所述，来自PYGB的circRNA可能通过与miR-338-3p竞争来调节SACC的发生和发展。
