Ju Rui, Huang Yanling, Guo Zeyou, Han Lu, Ji Suhui, Zhao Luyang, Long Jie
The State Key Laboratory of Oral Diseases, Sichuan University, 14, The 3rd Section of South People's Road, Chengdu, 610041, Sichuan, China.
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
Mol Cell Biochem. 2021 Feb;476(2):1269-1282. doi: 10.1007/s11010-020-03989-z. Epub 2020 Nov 25.
In order to reveal circular RNAs (circRNAs) differential expression profiles and investigate the function and mechanism of circRNAs in the metastasis of salivary adenoid cystic carcinoma (SACC), microarray was used to detect differentially expressed circRNAs in SACC-83 and SACC-lung metastasis (LM) cell lines. Up-regulated circRNAs were analyzed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to further predict their function. Expression of candidate circRNA and microRNA (miRNA) was determined using quantitative real-time polymerase chain reaction (qRT-PCR). Constructed circRNA-miRNA-mRNA co-expression network was based on TargetScan, miRanda databases. Wound healing and transwell assays were completed to examine the effects of hsa_circRNA_001982 and miR-181a-5p on cell migration and invasion. qRT-PCR confirmed hsa_circRNA_092556, hsa_circRNA_101379, and hsa_circRNA_001982 up-regulation in SACC-LM. miR-181a-5p was down-regulated in SACC-LM and correlated with up-regulated hsa_circRNA_001982. Wound healing and transwell assays indicated that silencing hsa_circRNA_001982 inhibited the migration and invasion of the SACC-LM cells. Furthermore, over-expression of hsa_circRNA_001982 promoted the migration and invasion of SACC-83 cells. Interestingly, up-regulation or down-regulation of miR-181a-5p led to the opposite result in wound healing and transwell assays. Overall, differential expression circRNA profiles in SACC-83 and SACC-LM cells may reveal potential targets and a novel mechanism of circRNAs in the metastasis of SACC. Moreover, the interaction of hsa_circRNA_001982/miR-181a-5p is closely related to the metastasis of SACC cells.
为了揭示环状RNA（circRNA）的差异表达谱，并研究circRNA在涎腺腺样囊性癌（SACC）转移中的功能和机制，采用基因芯片检测SACC-83和SACC肺转移（LM）细胞系中差异表达的circRNA。对上调的circRNA进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析，以进一步预测其功能。使用定量实时聚合酶链反应（qRT-PCR）检测候选circRNA和微小RNA（miRNA）的表达。基于TargetScan、miRanda数据库构建circRNA-miRNA-mRNA共表达网络。完成伤口愈合和Transwell实验，以检测hsa_circRNA_001982和miR-181a-5p对细胞迁移和侵袭的影响。qRT-PCR证实hsa_circRNA_092556、hsa_circRNA_101379和hsa_circRNA_001982在SACC-LM中上调。miR-181a-5p在SACC-LM中下调，并与上调的hsa_circRNA_001982相关。伤口愈合和Transwell实验表明，沉默hsa_circRNA_001982可抑制SACC-LM细胞的迁移和侵袭。此外，hsa_circRNA_001982的过表达促进了SACC-83细胞的迁移和侵袭。有趣的是，miR-181a-5p的上调或下调在伤口愈合和Transwell实验中导致相反的结果。总体而言，SACC-83和SACC-LM细胞中circRNA的差异表达谱可能揭示SACC转移中circRNA的潜在靶点和新机制。此外，hsa_circRNA_001982/miR-181a-5p的相互作用与SACC细胞的转移密切相关。
