Department of Stomatology, Longyan People's Hospital, Longyan, 364000, Fujian, China.
Department of Radiology, Longyan People's Hospital, No. 31, Denggao West Road, Xinluo District, Fujian Province, 364000, Longyan, China.
Clin Transl Oncol. 2023 Nov;25(11):3152-3164. doi: 10.1007/s12094-023-03182-w. Epub 2023 May 24.
Local recurrence, distant metastasis, and perineural invasion (PNI) viciously occur in salivary adenoid cystic carcinoma (SACC), resulting in a poor prognosis. This study aimed to explore the mechanism by which circular RNA RNF111 (circ-RNF111) regulates PNI in SACC by targeting the miR-361-5p/high mobility group box 2 (HMGB2) axis.
Circ-RNF111 and HMGB2 were highly expressed in SACC specimens, while miR-361-5p was underexpressed. Functional experiments showed that ablating circ-RNF111 or promoting miR-361-5p hindered the biological functions and PNI of SACC-LM cells.
HMGB2 overexpression induced the reversal of SACC-LM cell biological functions and PNI caused by circ-RNF111 knockout. Furthermore, reduction of circ-RNF111 suppressed PNI in a SACC xenograft model. Circ-RNF111 regulated HMGB2 expression through targeted modulation of miR-361-5p.
Taken together, circ-RNF111 stimulates PNI in SACC by miR-361-5p/HMGB2 axis and may serve as a potential therapeutic target for SACC.
局部复发、远处转移和神经周围侵犯（PNI）在涎腺腺样囊性癌（SACC）中恶性发生，导致预后不良。本研究旨在探讨环状 RNA RNF111（circ-RNF111）通过靶向 miR-361-5p/高迁移率族蛋白 2（HMGB2）轴调节 SACC 中 PNI 的机制。
circ-RNF111 和 HMGB2 在 SACC 标本中高表达，而 miR-361-5p 低表达。功能实验表明，circ-RNF111 的缺失或 miR-361-5p 的促进抑制了 SACC-LM 细胞的生物学功能和 PNI。
HMGB2 的过表达诱导了 circ-RNF111 敲除引起的 SACC-LM 细胞生物学功能和 PNI 的逆转。此外，circ-RNF111 的减少在 SACC 异种移植模型中抑制了 PNI。circ-RNF111 通过靶向调节 miR-361-5p 来调节 HMGB2 的表达。
总之，circ-RNF111 通过 miR-361-5p/HMGB2 轴刺激 SACC 中的 PNI，可能是 SACC 的潜在治疗靶点。
