Yunusova R Yu, Gorelova V G, Kombarova S Yu, Aleshkin A V, Efimova O G, Bichucher A M, Martynenko I G, Alieva E R
The Federal Budget Institution of Science "G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology" of Rospotrebnadzor of Russia, 125212, Moscow, Russia.
The State Budget Educational Institution of Higher Professional Education "The Dagestan State Medical Academy" of Minzdrav of Russia, 367016, Makhachkala, Russia.
Klin Lab Diagn. 2018;63(2):110-113. doi: 10.18821/0869-2084-2018-63-2-110-113.
The purpose of study is to develop a traditional Endo's growth medium with the view of suppression of swarming of Proteus vulgaris and Proteus mirabilis. The object of study was a commercial dry Endo's growth medium. The inoculation of reference strains of enterobacteria and clinical sample (feces, urine, phlegm) was implemented according the normative documents. It is demonstrated that brining into Endo's growth medium tryptophan and sodium salts of bile acids in a particular ratio of their concentrations suppressed swarming of Proteus vulgaris and Proteus mirabilis. The obtained results demonstrated that application of enhanced Endo's growth medium significantly increases its differential and diagnostic characteristics.
本研究的目的是开发一种传统的远藤氏培养基，以期抑制普通变形杆菌和奇异变形杆菌的群游现象。研究对象为市售的远藤氏培养基干粉。根据规范性文件对肠杆菌参考菌株和临床样本（粪便、尿液、痰液）进行接种。结果表明，以特定浓度比例向远藤氏培养基中添加色氨酸和胆汁酸钠盐可抑制普通变形杆菌和奇异变形杆菌的群游现象。所得结果表明，使用改良的远藤氏培养基可显著提高其鉴别和诊断特性。
