Koh Byumseok, Jeon Hyojin, Kim Dahee, Kang Dukjin, Kim Kwang Rok
Bio Platform Technology Research Center, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Innovative Target Research Center, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Oncol Lett. 2019 Feb;17(2):2409-2417. doi: 10.3892/ol.2018.9836. Epub 2018 Dec 14.
Interactions between cancer cells and the surrounding fibroblasts serve an important role in cancer proliferation. Colon cancer co-culture model with colon fibroblasts and two metastatic models with lung and skin fibroblasts were established, and the co-culture effects on colon cancer cell proliferation, apoptosis and drug response were evaluated. Co-culture with CCD-18Co and BJ reduces SW480 cell proliferation by 4.2 and 5.3%, respectively, while WI-38 acts as a positive regulator and increases SW480 cell proliferation by 36%. CCD-18Co and BJ co-culture can also enhance XAV939 potency against SW480 cells by 16.8 and 27.3%; however, WI-38 co-culture reduces the effect of XAV939 by 38.2%. The present results suggest that, depending on fibroblast type, co-culture can have a positive/negative influence on colon cancer growth; therefore, care should be taken when considering fibroblasts as a target for future cancer therapies.
癌细胞与周围成纤维细胞之间的相互作用在癌症增殖中发挥重要作用。建立了结肠癌细胞与结肠成纤维细胞的共培养模型以及与肺和皮肤成纤维细胞的两种转移模型，并评估了共培养对结肠癌细胞增殖、凋亡和药物反应的影响。与CCD-18Co和BJ共培养分别使SW480细胞增殖降低4.2%和5.3%，而WI-38则作为正向调节因子，使SW480细胞增殖增加36%。CCD-18Co和BJ共培养还可使XAV939对SW480细胞的效力分别增强16.8%和27.3%；然而，WI-38共培养使XAV939的作用降低38.2%。目前的结果表明，根据成纤维细胞类型，共培养可对结肠癌生长产生正向/负向影响；因此，在将成纤维细胞作为未来癌症治疗靶点时应谨慎考虑。
