Barai Pallob, Hossain Khondoker Moazzem, Rahman Sana Mohammad Mahbubur, Al Mazid Mohammad Faysal, Gazi Mohammad Shamim
Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
Prev Nutr Food Sci. 2018 Dec;23(4):294-300. doi: 10.3746/pnf.2018.23.4.294. Epub 2018 Dec 31.
Probiotics may offer a safe intervention for diarrheal diseases. The aim of the present study was the assessment of the antidiarrheal property of probiotic bacteria. For their antidiarrheal efficacy assessment, yogurt was prepared using the isolated bacteria from selective regional yogurt of Bangladesh, and mice model trails were conducted using castor oil induced diarrheal mice. The probiotic treatment was applied on three mice groups, each having 6 mice and their respective doses were 50 mL/kg body weight in treatment group (TG) 1, 100 mL/kg body weight in TG2, and 150 mL/kg body weight in TG3. A four week treatment of probiotic significantly (<0.001) reduced the percentage (67.37%) of diarrhea in TG3 (150 mL yogurt/kg body weight). All the treatment groups showed a significant (<0.001) increase in the latent periods, reduced the total fecal output, and frequency and fecal water content compared to the negative control group. Serum electrolytes (Na and K) and total protein levels were higher in the TG3 compared to the negative control group. Further research regarding molecular characterization and identification of specific genes and proteins of interest may help to develop the next generation bacteriocins and antidiarrheal drugs.
益生菌可能为腹泻疾病提供一种安全的干预措施。本研究的目的是评估益生菌的止泻特性。为评估其止泻效果，使用从孟加拉国特定地区酸奶中分离出的细菌制备酸奶，并对蓖麻油诱导腹泻的小鼠进行小鼠模型试验。将益生菌处理应用于三个小鼠组，每组有6只小鼠，其各自的剂量在治疗组（TG）1中为50 mL/kg体重，在TG2中为100 mL/kg体重，在TG3中为150 mL/kg体重。四周的益生菌治疗显著（<0.001）降低了TG3（150 mL酸奶/kg体重）中腹泻的百分比（67.37%）。与阴性对照组相比，所有治疗组的潜伏期均显著（<0.001）延长，粪便总排出量、频率和粪便含水量均降低。与阴性对照组相比，TG3中的血清电解质（钠和钾）和总蛋白水平更高。关于感兴趣的特定基因和蛋白质的分子特征鉴定的进一步研究可能有助于开发下一代细菌素和止泻药物。
