Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Beijing 100097, China.
Centre of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Plant Dis. 2017 Dec;101(12):2123-2136. doi: 10.1094/PDIS-12-16-1741-RE. Epub 2017 Oct 9.
Grape white rot is a common disease and causes considerable yield losses in many grape-growing regions when environmental conditions are favorable. We surveyed grape white rot in five provinces in China and collected 27 isolates from diseased grape tissues. Multigene phylogenetic analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2), the 28S large subunit of nuclear ribosomal RNA (LSU), partial translation elongation factor 1-alpha gene (TEF 1-α), and partial histone 3 gene (HIS), coupled with genealogical concordance phylogenetic species recognition and morphological observations, revealed that Coniella vitis sp. nov. and C. diplodiella are the causal agents of grape white rot in China. Koch's postulates were performed on Vitis vinifera cv. Summer Black in a greenhouse. These results confirmed the pathogenicity on grapes, as symptoms were reproduced, and also indicated significant variations in the virulence among C. vitis isolates. This work provides evidence that C. vitis is the main pathogen of grape white rot in China and also provides an optimized multigene backbone for resolving Coniella species.
葡萄白腐病是一种常见病害，当环境条件适宜时，会在许多葡萄种植区造成相当大的产量损失。我们在中国五个省份调查了葡萄白腐病，并从患病的葡萄组织中收集了 27 个分离株。通过对内转录间隔区（ITS1-5.8S-ITS2）、核核糖体 RNA 大亚基（LSU）、部分翻译延伸因子 1-α 基因（TEF1-α）和部分组蛋白 3 基因（HIS）的多基因系统发育分析，结合系统发育物种识别和形态学观察，表明中国葡萄白腐病的病原菌为葡萄钩丝壳菌和二孢钩丝壳菌。在温室中对 Vitis vinifera cv. Summer Black 进行了柯赫氏假设验证。这些结果证实了葡萄上的致病性，症状得以重现，同时也表明葡萄钩丝壳菌分离株的毒力存在显著差异。这项工作为葡萄白腐病在中国的主要病原菌是葡萄钩丝壳菌提供了证据，也为解决钩丝壳属物种提供了优化的多基因骨干。
