Comparative genomic and functional analyses of ZBSF16 with biocontrol potential against grapevine diseases, provide insights into its genes related to plant growth-promoting and biocontrol mechanisms.

is a plant growth-promoting rhizobacteria (PGPR) widely distributed in various environments. ZBFS16 was isolated from the wheat rhizosphere and significantly suppressed grape white rot disease caused by . Here, we present the complete genome sequence of ZBFS16, which consists of a 5.83 Mb circular chromosome with an average G + C content of 45.62%. Phylogenetic analyses showed that ZBFS16 belongs to the genus and was similar to ZF390, HS311 and HJ-2. Comparative analysis with three closely related sequenced strains of identified the conservation of genes involved in indole-3-acetic acid production, phosphate solubilization, nitrogen fixation, biofilm formation, flagella and chemotaxis, quorum-sensing systems, two-component systems, antimicrobial substances and resistance inducers. Meanwhile, experiments were also performed to confirm these functions. In addition, the strong colonization ability of ZBFS16 was observed in soil, which provides it with great potential for use in agriculture as a PGPR. This study will be helpful for further studies of on the mechanisms of plant growth promotion and biocontrol.