Ibrahim Yasser E, Saleh Amgad A, Al-Saleh Mohammed A
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia, and Plant Pathology Research Institute, Agriculture Research Center, Giza, Egypt.
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia, and Agricultural Genetic Engineering Research Institute, Agriculture Research Center, Giza, Egypt.
Plant Dis. 2017 May;101(5):761-765. doi: 10.1094/PDIS-08-16-1213-RE. Epub 2017 Feb 24.
Applications of formulated bacteriophages with skim milk and sucrose or nonformulated bacteriophages combined with acibenzolar-S-methyl (ASM) were compared with copper bactericides applications for suppressing Asiatic citrus canker (ACC) caused by Xanthomonas citri subsp. citri (Xcc) on leaves under greenhouse and field conditions in Saudi Arabia. Bacteriophages were applied one day prior to inoculation of Mexican lime (Citrus aurantifolia) plants with Xcc, then twice a week until the end of the trials. Copper hydroxide was applied once prior to inoculation and then every seven days afterward, whereas ASM was applied one week prior to inoculation and then every 21 days afterward. Under greenhouse conditions, the incidence of ACC on leaves was reduced significantly from 75.2 to 12.8% or 18.3% for plants treated with copper hydroxide or bacteriophages in combination with ASM, respectively. Applications of formulated phages in combination with ASM as soil drench under field conditions significantly decreased disease incidence by 14.8% (Trial 1) and 16.8% (Trial 2) compared with untreated control plants. Overall, the Xcc-inoculated plants treated with bacteriophages + ASM combination showed significant ACC reduction under greenhouse and field conditions. The bacteriophages + ASM combination tested in these trials can be an effective tool in the integrated management programs of Asiatic citrus canker disease.
在沙特阿拉伯的温室和田间条件下，将添加脱脂牛奶和蔗糖的配方噬菌体或与苯并噻二唑 - S - 甲基（ASM）组合的非配方噬菌体的应用，与铜杀菌剂的应用进行比较，以抑制由柑橘溃疡病菌（Xanthomonas citri subsp. citri，Xcc）引起的亚洲柑橘溃疡病（ACC）在叶片上的发生。在向墨西哥莱檬（Citrus aurantifolia）植株接种Xcc的前一天施用噬菌体，然后每周两次，直至试验结束。在接种前施用一次氢氧化铜，之后每七天施用一次，而ASM在接种前一周施用一次，之后每21天施用一次。在温室条件下，用氢氧化铜或与ASM组合的噬菌体处理的植株，叶片上ACC的发病率分别从75.2%显著降低至12.8%或18.3%。在田间条件下，作为土壤浇灌剂施用配方噬菌体与ASM的组合，与未处理的对照植株相比，显著降低了发病率，在试验1中降低了14.8%，在试验2中降低了16.8%。总体而言，在温室和田间条件下，用噬菌体 + ASM组合处理接种Xcc的植株，ACC发病率显著降低。在这些试验中测试的噬菌体 + ASM组合可以成为亚洲柑橘溃疡病综合管理计划中的一种有效工具。
