College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin, 130118, China.
College of Chinese Medicinal Materials, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin, 130118, China.
Sci Rep. 2019 Jan 24;9(1):615. doi: 10.1038/s41598-018-36349-5.
Ginseng, Panax ginseng C.A. Meyer, is one of the most important medicinal herbs for human health and medicine in which ginsenosides are known to play critical roles. The genes from the cytochrome P450 (CYP) gene superfamily have been shown to play important roles in ginsenoside biosynthesis. Here we report genome-wide identification of the candidate PgCYP genes for ginsenoside biosynthesis, development of functional SNP markers for its manipulation and systems analysis of its underlying molecular mechanism. Correlation analysis identified 100 PgCYP genes, including all three published ginsenoside biosynthesis PgCYP genes, whose expressions were significantly correlated with the ginsenoside contents. Mutation association analysis identified that six of these 100 PgCYP genes contained SNPs/InDels that were significantly associated with ginsenosides biosynthesis (P ≤ 1.0e-04). These six PgCYP genes, along with all ten published ginsenoside biosynthesis genes from the PgCYP and other gene families, formed a strong co-expression network, even though they varied greatly in spatio-temporal expressions. Therefore, this study has identified six new ginsenoside biosynthesis candidate genes, provided a genome-wide insight into how they are involved in ginsenoside biosynthesis and developed a set of functional SNP markers useful for enhanced ginsenoside biosynthesis research and breeding in ginseng and related species.
人参，Panax ginseng C.A. Meyer，是人类健康和医学中最重要的药用植物之一，其中人参皂苷被认为在其中发挥着关键作用。细胞色素 P450（CYP）基因超家族的基因已被证明在人参皂苷生物合成中发挥着重要作用。在这里，我们报告了对参与人参皂苷生物合成的候选 PgCYP 基因进行全基因组鉴定，开发了用于其操作的功能 SNP 标记，并对其潜在分子机制进行了系统分析。相关性分析确定了 100 个 PgCYP 基因，包括所有三个已发表的人参皂苷生物合成 PgCYP 基因，它们的表达与人参皂苷含量显著相关。突变关联分析确定，这 100 个 PgCYP 基因中的 6 个基因含有与人参皂苷生物合成显著相关的 SNP/InDels（P≤1.0e-04）。这六个 PgCYP 基因与其他基因家族中的所有十个已发表的人参皂苷生物合成基因一起，形成了一个强大的共表达网络，尽管它们在时空表达上差异很大。因此，本研究鉴定了六个新的人参皂苷生物合成候选基因，为它们如何参与人参皂苷生物合成提供了全基因组的见解，并开发了一组功能 SNP 标记，可用于增强人参和相关物种的人参皂苷生物合成研究和育种。
