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对四个物种的转录组和代谢组进行比较分析，以探索代谢物生物合成的动态过程。

Comparative transcriptome and metabolome analyses of four species explore the dynamics of metabolite biosynthesis.

作者信息

Koo Hyunjin, Lee Yun Sun, Nguyen Van Binh, Giang Vo Ngoc Linh, Koo Hyun Jo, Park Hyun-Seung, Mohanan Padmanaban, Song Young Hun, Ryu Byeol, Kang Kyo Bin, Sung Sang Hyun, Yang Tae-Jin

机构信息

Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.

Department of Agricultural Biotechnology, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.

出版信息

J Ginseng Res. 2023 Jan;47(1):44-53. doi: 10.1016/j.jgr.2022.07.001. Epub 2022 Jul 16.

DOI:10.1016/j.jgr.2022.07.001
PMID:36644396
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9834023/
Abstract

BACKGROUND

The genus in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between species has influenced their biosynthetic pathways is not fully understood.

METHODS

Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species ( and ) and two diploid species ( and ) revealed the diversity of their metabolites and related gene expression profiles.

RESULTS

The transcriptome analysis showed that (s) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of s contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species.

CONCLUSION

These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the genus.

摘要

背景

五加科人参属植物在全球范围内一直被用作传统药用植物，已知其能生物合成人参皂苷和植物甾醇。然而，人参属物种间的遗传变异如何影响其生物合成途径尚未完全明确。

方法

对两种四倍体物种（人参和三七）以及两种二倍体物种（西洋参和竹节参）不定根的转录组和代谢组进行同步分析，揭示了它们代谢产物的多样性以及相关基因表达谱。

结果

转录组分析表明，参与植物甾醇生物合成的基因在二倍体物种中上调，而有助于人参皂苷生物合成的基因在四倍体物种中表达更高。与这些结果一致，相对于二倍体物种，四倍体物种中达玛烷二醇型人参皂苷的含量更高。

结论

这些结果表明，全基因组复制事件在四倍体人参属物种的进化或生态适应过程中影响了三萜生物合成途径。本研究为进一步探索人参属的遗传变异提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/b6e0d7abdb38/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/8b1fda29f40c/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/d1354a6e084c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/a51bf697cb90/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/9fe57f56052e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/1cb102afef24/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/b6e0d7abdb38/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/8b1fda29f40c/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/d1354a6e084c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/a51bf697cb90/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/9fe57f56052e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/1cb102afef24/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15b7/9834023/b6e0d7abdb38/gr5.jpg

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