Sivagurunathan U, Srivastava Prem Prakash, Gupta Subodh, Krishna Gopal
Fish Nutrition, Biochemistry and Physiology Division, ICAR - Central Institute of Fisheries Education (Deemed University), Off Yari Road, Panch Marg, Versova, Mumbai 400 061, India.
Saudi J Biol Sci. 2020 Dec;27(12):3593-3600. doi: 10.1016/j.sjbs.2020.07.033. Epub 2020 Aug 3.
This study assessed the responses of vitamin-D intraperitoneally injected to Rohu, @ of 0 IU/kg bw (only solvent), 100 IU/kg bw and 500 IU/kg bw reared in 20 and 40 ppm of calcium (Ca) enriched water. The cellular changes in Corpuscles of Stannius (CS) gland, serum Ca, and inorganic phosphate (P) level were analysed up to the 60th day. Rohu administered with 100 IU/kg bw D and exposed to 40 ppm Ca-rich water exhibited notable hyperplasia of CS compared with their control groups. Notable changes with high serum Ca level (13.87 ± 0.3 mg/dl) was detected on the 5th day in fish exposed to 40 ppm Ca-rich water, while related values attained (13.74 ± 0.1 mg/dl) only after 7 days in 20 ppm Ca-rich water of 500 IU/kg bw vitamin D injection. Similarly, high serum P level (7.66 ± 0.2 mg/dl) in 40 ppm Ca injected with D at 500 IU/kg bw. The results demonstrated that the Ca homeostasis of is influenced by intra-peritoneal vitamin D. Progressive studies should be conducted by increasing the dose of vitamin D to investigate optimum dose/supplement in feed for commercially important aquaculture teleost for maximum and sustainable absorption of Ca from the variable water Calcium levels to maintain Ca homeostasis.
本研究评估了腹腔注射维生素D对在富含20 ppm和40 ppm钙（Ca）的水中饲养的印度鲤（Rohu）的影响，剂量分别为0 IU/kg体重（仅溶剂）、100 IU/kg体重和500 IU/kg体重。在第60天之前分析了斯坦尼斯小体（CS）腺的细胞变化、血清钙和无机磷（P）水平。与对照组相比，腹腔注射100 IU/kg体重维生素D并暴露于40 ppm富钙水中的印度鲤CS出现明显增生。在暴露于40 ppm富钙水中的鱼中，第5天检测到血清钙水平显著升高（13.87±0.3 mg/dl），而在注射500 IU/kg体重维生素D的20 ppm富钙水中，相关值在7天后才达到（13.74±0.1 mg/dl）。同样，在注射500 IU/kg体重维生素D的40 ppm钙水中，血清磷水平较高（7.66±0.2 mg/dl）。结果表明，腹腔注射维生素D会影响印度鲤的钙稳态。应通过增加维生素D剂量进行进一步研究，以调查商业上重要的水产养殖硬骨鱼饲料中的最佳剂量/补充剂，以便从不同的水体钙水平中最大程度地可持续吸收钙，从而维持钙稳态。
