Meng Yuanyuan, Li Qi, Li Lianwei, Ma Rong
Department of Gynaecology, Harbin Medical University Cancer Hospital, 150 HaPing Road, Nangang District, Harbin 150001, Heilongjiang, P.R. China.
Department of Radiotherapy for Gynaecology, Harbin Medical University Cancer Hospital, Harbin 150001, Heilongjiang, P.R. China.
Biol Chem. 2017 Dec 20;399(1):93-100. doi: 10.1515/hsz-2017-0199.
This study was intended to analyze effects of lncRNA CRNDE on cervical cancer cell growth and metastasis. Fifty pairs of cervical cancer tissues and corresponding adjacent tissues were collected. Expressions of long non-coding RNAs (lncRNAs) in tissue samples were detected by microarray analysis. Expression levels of CRNDE in cervical cancer cells and normal cells were detected by qRT-PCR. Cell-counting kit-8 (CCK-8) assay and clone formation assay were utilized to evaluate cell growth. Wound healing assay and Transwell assay were conducted to detect the migratory and invasive capability of cervical cancer cells. The expressions of CRNDE in cervical cancer tissues and cells were higher than those in normal tissues and cells. CCK-8 assay and clone formation assay showed that the knockdown of CRNDE could inhibit the cell proliferation of HeLa and C-33A cells. Wound healing assay indicated that the downregulation of CRNDE expression could suppress the cell migration. The result of a Transwell assay demonstrated that the number of invasion cells reduced in the CRNDE-si group in comparison with the Mock group. LncRNA CRNDE could promote the cell growth and stimulate the metastasis of cervical cancer cells.
本研究旨在分析长链非编码RNA（lncRNA）CRNDE对宫颈癌细胞生长和转移的影响。收集了50对宫颈癌组织及相应的癌旁组织。通过基因芯片分析检测组织样本中长链非编码RNA（lncRNAs）的表达。采用实时定量聚合酶链反应（qRT-PCR）检测宫颈癌细胞和正常细胞中CRNDE的表达水平。利用细胞计数试剂盒-8（CCK-8）法和克隆形成试验评估细胞生长情况。进行伤口愈合试验和Transwell试验以检测宫颈癌细胞的迁移和侵袭能力。CRNDE在宫颈癌组织和细胞中的表达高于正常组织和细胞。CCK-8法和克隆形成试验表明，敲低CRNDE可抑制HeLa和C-33A细胞的增殖。伤口愈合试验表明，下调CRNDE表达可抑制细胞迁移。Transwell试验结果显示，与Mock组相比，CRNDE-si组侵袭细胞数量减少。lncRNA CRNDE可促进宫颈癌细胞的生长并刺激其转移。
