Sohn Dong-Seok, Moon Yong-Suk
Department of Dentistry and Oral and Maxillofacial Surgery, Daegu Catholic University Medical Center, Daegu, Korea.
Department of Anatomy, Catholic University of Daegu School of Medicine, Daegu, Korea.
Anat Cell Biol. 2018 Dec;51(Suppl 1):S1-S12. doi: 10.5115/acb.2018.51.S1.S1. Epub 2018 Dec 31.
The purpose of this animal study is to evaluate, by histomorphometric analysis, bone regeneration in rabbit's maxillary sinuses with blood clots alone, Bio-Oss, β-tricalcium phosphate (β-TCP), and demineralized tooth dentin (DTD) grafting. Bilateral sinus augmentation procedures were performed in 18 adult male rabbits. Rectangular replaceable bony windows were made with a piezoelectric thin saw insert. In the group 1, blood clots were filled; group 2, anorganic bovine graft (Bio-Oss) was grafted; group 3, β-TCP was grafted; group 4, DTD was grafted, and covered by replaceable bony windows. Animals were sacrificed at 2, 4, and 8 weeks after surgical procedure. The augmented sinuses were evaluated by histomorphometric analysis using hematoxylin and eosin and Masson's trichrome stains. Histologically, new bone formation was revealed along the elevated sinus membrane and all graft materials. The new bone area of the group 2 was significantly greater than the group 1, and of the group 3 was significantly greater than the group 2, and of the group 4 was significantly greater than the group 3 at 8 weeks with <0.05. The bone marrow area of group 1 was significantly greater than other groups at 8 weeks. The DTD area was significantly lesser than Bio-Oss or β-TCP particles area at 8 weeks. This present study suggests that DTD can be effective graft materials for bone regeneration of the maxillary sinus augmentation.
本动物研究的目的是通过组织形态计量分析，评估单独使用血凝块、Bio-Oss、β-磷酸三钙（β-TCP）和脱矿牙本质（DTD）移植时兔上颌窦的骨再生情况。对18只成年雄性兔子进行双侧鼻窦增大手术。使用压电薄锯片制作矩形可替换骨窗。第1组填充血凝块；第2组植入无机牛骨移植物（Bio-Oss）；第3组植入β-TCP；第4组植入DTD，并用可替换骨窗覆盖。在手术后2周、4周和8周处死动物。使用苏木精和伊红以及马松三色染色通过组织形态计量分析评估增大的鼻窦。组织学上，在抬高的鼻窦黏膜和所有移植材料周围均可见新骨形成。在8周时，第2组的新骨面积显著大于第1组，第3组显著大于第2组，第4组显著大于第3组，P<0.05。在8周时，第1组的骨髓面积显著大于其他组。在8周时，DTD面积显著小于Bio-Oss或β-TCP颗粒面积。本研究表明，DTD可作为上颌窦增大骨再生的有效移植材料。
