Postgraduate Student, Department of Dentistry and Oral and Maxillofacial Surgery, School of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
Professor and Chairman, Department of Dentistry and Oral and Maxillofacial Surgery, Catholic University Hospital of Daegu, Daegu, Republic of Korea.
Implant Dent. 2018 Jun;27(3):324-331. doi: 10.1097/ID.0000000000000755.
The aim of this animal study is to evaluate, by histomorphometric analysis, new bone formation in rabbit maxillary sinuses with Bio-Oss and demineralized particulate human tooth graft.
Bilateral sinus augmentation procedures were performed in 8 adult male rabbits. After preparation of replaceable bony windows on the lateral wall of the nasal cavity with a piezoelectric surgical device, deproteinized bovine graft (Bio-Oss) was grafted in the new compartment of the maxillary sinus after elevation of the sinus membrane in the control group. In the experimental group, the demineralized human particulate tooth bone was grafted in the sinus. The replaceable bony window was repositioned over the bone graft in both groups. Animals were killed at 2 and 8 weeks after the surgical procedure. The augmented sinuses were evaluated by histomorphometric analysis using hematoxylin-eosin and Masson trichrome stains.
Histologically, new bone was revealed along the elevated sinus membrane and both bone grafts. In the control group, the new bone area at 8 weeks was not significantly different than that at 2 weeks. In the experimental group, the new bone area at 8 weeks was significantly greater than that at 2 weeks.
Significant higher new bone formation was revealed in the experimental group than in the control group.
本动物研究旨在通过组织形态计量分析，评估 Bio-Oss 和脱矿人牙颗粒移植物在兔上颌窦中的新骨形成。
8 只成年雄性兔进行双侧鼻窦增强术。在用压电手术装置制备鼻腔侧壁可替换骨窗后，在对照组中提升窦膜后将脱蛋白牛移植物（Bio-Oss）移植到上颌窦的新腔室中。在实验组中，将脱矿人牙颗粒骨移植到窦内。两组均在骨移植物上重新定位可替换的骨窗。手术后 2 周和 8 周处死动物。通过苏木精-伊红和 Masson 三色染色的组织形态计量分析评估增强的窦。
组织学上，新骨沿着升高的窦膜和两种移植物显露。在对照组中，8 周时的新骨面积与 2 周时无显著差异。在实验组中，8 周时的新骨面积明显大于 2 周时。
实验组的新骨形成明显高于对照组。
