School of Life Sciences, Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
Nat Prod Rep. 2019 Sep 18;36(9):1237-1248. doi: 10.1039/c8np00081f.
Covering: up to December 2018 This article aims to highlight advantages, drawbacks and issues that users should consider when implementing the use of CRISPR/Cas9-tools for genome editing in streptomycetes, the most prolific source of antimicrobial natural products to date. Here, we examine four toolkits that have so far been made available for streptomycete in vivo-engineering and one for in vitro-editing, and review how they have been applied over the last three years. Our critical evaluation of these toolkits intends to support potential users in determining what they could achieve, what they should consider and what system they should select/optimise for their application.
截至 2018 年 12 月 本文旨在强调在链霉菌中使用 CRISPR/Cas9 工具进行基因组编辑时的优点、缺点和用户应考虑的问题，链霉菌是迄今为止抗菌天然产物的最丰富来源。在这里，我们研究了迄今为止可用于链霉菌体内工程的四个工具包和一个体外编辑工具包，并回顾了它们在过去三年中的应用。我们对这些工具包的批判性评估旨在支持潜在用户确定他们可以实现什么，他们应该考虑什么以及他们应该选择/优化哪个系统来进行他们的应用。
