Departent of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O.Box: 196, Gondar, Ethiopia.
National Institute for Public Health and the Environment (RIVM), 3720, BA, Bilthoven, The Netherlands.
BMC Infect Dis. 2019 Jan 25;19(1):92. doi: 10.1186/s12879-019-3712-1.
Previous studies demonstrated that the diversity and composition of respiratory microbiota in TB patients were different from healthy individuals. Therefore, the aim of the present analysis was to estimate the relative proportion of respiratory microbiota at phylum and genus levels among TB cases and healthy controls.
The PubMed and Google Scholar online databases were searched to retrieve relevant studies for the analysis. The statistical analysis was done using STATA version 11, pooled estimates are presented using graphs. The summary of findings in included studies is also presented in Table 1.
The phylum level analysis shows that the pooled proportions of Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Crenarchaeota were determined among tuberculosis patients and healthy controls. In brief, Firmicutes, and Proteobacteria were the most abundant bacterial phyla in both TB cases and healthy controls, composing 39.9 and 22.7% in TB cases and 39.4 and 19.5% in healthy controls, respectively. The genus level analysis noted that Streptococcus (35.01%), Neisseria (27.1%), Prevotella (9.02%) and Veillonella (7.8%) were abundant in TB patients. The Prevotella (36.9%), Gammaproteobacteria (22%), Streptococcus (19.2%) and Haemophilus (15.4%) were largely seen in healthy controls. Interestingly, Veillonella, Rothia, Leuconostoc were unique to TB cases, whereas Lactobacillus, and Gammaproteobacteria, Haemophilus, and Actinobacillus were identified only in healthy controls.
The composition of the respiratory microbiota in TB patients and healthy controls were quite different. More deep sequencing studies are needed to explore the microbial variation in the respiratory system in connection with TB.
先前的研究表明，肺结核患者的呼吸微生物群落多样性和组成与健康个体不同。因此，本分析旨在估计肺结核病例和健康对照者在门和属水平的呼吸微生物群落的相对比例。
通过 PubMed 和 Google Scholar 在线数据库检索相关研究进行分析。使用 STATA 版本 11 进行统计分析，通过图表呈现汇总估计值。表 1 还呈现了纳入研究的发现总结。
门水平分析表明，在肺结核患者和健康对照者中确定了厚壁菌门、变形菌门、拟杆菌门、放线菌门和泉古菌门的比例。简而言之，Firmicutes 和 Proteobacteria 是肺结核病例和健康对照者中最丰富的细菌门，分别占 39.9%和 22.7%，占 39.4%和 19.5%。属水平分析表明，在肺结核患者中丰富的细菌属有链球菌属（35.01%）、奈瑟菌属（27.1%）、普雷沃氏菌属（9.02%）和韦荣球菌属（7.8%）。在健康对照者中丰富的细菌属有普雷沃氏菌属（36.9%）、γ-变形菌纲（22%）、链球菌属（19.2%）和嗜血杆菌属（15.4%）。有趣的是，韦荣球菌属、罗氏菌属和勒克菌属是肺结核病例所特有的，而乳杆菌属、γ-变形菌纲、嗜血杆菌属和放线杆菌属仅在健康对照者中发现。
肺结核患者和健康对照者的呼吸微生物群落组成差异较大。需要更多的深度测序研究来探索与肺结核相关的呼吸系统微生物变异。
