Hong Bo-Young, Paulson Joseph N, Stine O Colin, Weinstock George M, Cervantes Jorge L
The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Genentech, Department of Biostatistics, Product Development, South San Francisco, CA, USA.
Tuberculosis (Edinb). 2018 Mar;109:102-108. doi: 10.1016/j.tube.2018.02.006. Epub 2018 Feb 22.
The lung microbiota has received less attention compared to other body sites, in part because its study carries special technological difficulties related to obtaining reliable samples as compared to other body niches. The limited number of studies on the sputum microbiota on TB patients and controls available so far have reported inconsistent, and sometimes, contradictory results. Aiming to clarify if changes in the lung microbiota composition are associated with pulmonary TB, we performed a meta-analysis of available data on microbiota of the lower respiratory tract in TB patients and healthy controls. Re-processing next generation sequencing data under uniform parameters and utilizing state-of-the-art bioinformatics analysis, we obtained distinct clusterings of microbiota between TB cases vs. controls across multiple studies. We identified Tumebacillus ginsengisoli, Propionibacterium acnes, Haemophilus parahaemolyticus as differentially abundant species signature in healthy controls while Caulobacter henricii, Actinomyces graevenitzii, Rothia mucilaginosa, in addition to Mycobacterium tuberculosis as differentially abundant species signature in TB cases, and described R. mucilaginosa as the anchoring species in a network of bacteria co-occurring with Mycobacterium tuberculosis (Mtb) infection.
与身体的其他部位相比，肺部微生物群受到的关注较少，部分原因是与其他身体微环境相比，对其进行研究存在与获取可靠样本相关的特殊技术困难。迄今为止，关于肺结核患者和对照者痰液微生物群的研究数量有限，报告的结果不一致，有时甚至相互矛盾。为了阐明肺部微生物群组成的变化是否与肺结核相关，我们对肺结核患者和健康对照者下呼吸道微生物群的现有数据进行了荟萃分析。在统一参数下重新处理下一代测序数据，并利用最先进的生物信息学分析，我们在多项研究中获得了肺结核病例与对照者之间微生物群的不同聚类。我们确定人参肿瘤杆菌、痤疮丙酸杆菌、副溶血嗜血杆菌是健康对照者中差异丰富的物种特征，而亨利氏柄杆菌、格氏放线菌、黏液罗氏菌，以及结核分枝杆菌是肺结核病例中差异丰富的物种特征，并将黏液罗氏菌描述为与结核分枝杆菌（Mtb）感染共同出现的细菌网络中的锚定物种。
