Department of Nephrology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, Guangdong, China.
Department of Nephrology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, Guangdong, China.
Life Sci. 2019 Feb 15;219:353-363. doi: 10.1016/j.lfs.2019.01.032. Epub 2019 Jan 23.
Artemisinin (Art) plays a renoprotective role in diabetic nephropathy (DN) rats. However, the differential gene expression profile and underlying molecular mechanism of Art treatment in DN is not well understood. We constructed an animal model of DN by injection of streptozotocin (STZ) in rats. We then examined the profile of differentially expressed genes following administration of Art using RNA-sequencing (KANGCH&EN, Shanghai, China). Five genes identified by RNA-sequencing were randomly selected and validated by qRT-PCR. Bioinformatic analyses were performed to study these differentially expressed genes. We identified a total of 31 genes that were significantly up-regulated in DN samples compared to both normal and Art treatment samples, and 38 genes that were significantly down-regulated in DN samples compared to both normal and Art treatment samples. The identified genes were associated with a list of gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and may be involved in the mechanism underlying Art treatment of DN. Thus, the results from the current study demonstrate that genes are aberrantly expressed after Art treatment and identify promising targets in the treatment of DN with artemisinin.
青蒿素（Art）在糖尿病肾病（DN）大鼠中发挥肾保护作用。然而，青蒿素治疗 DN 的差异基因表达谱和潜在分子机制尚不清楚。我们通过向大鼠注射链脲佐菌素（STZ）构建了 DN 动物模型。然后，我们使用 RNA 测序（KANGCH&EN，上海，中国）检查了 Art 给药后的差异表达基因谱。通过 qRT-PCR 随机验证了 RNA 测序鉴定的 5 个基因。进行了生物信息学分析来研究这些差异表达的基因。我们总共鉴定出 31 个在 DN 样本中与正常和 Art 治疗样本相比显著上调的基因，以及 38 个在 DN 样本中与正常和 Art 治疗样本相比显著下调的基因。鉴定出的基因与基因本体论（GO）术语和京都基因与基因组百科全书（KEGG）途径列表相关，可能参与了 Art 治疗 DN 的机制。因此，本研究的结果表明，Art 治疗后基因表达异常，并确定了用青蒿素治疗 DN 的有前途的靶点。
