National Centre for Epidemiology and Population Health, Research School of Population Health, The Australian National University, Canberra, Australia; Departments of General Medicine and Infectious Diseases, The Canberra Hospital, Canberra, Australia.
College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.
J Invest Dermatol. 2019 Jul;139(7):1545-1553.e6. doi: 10.1016/j.jid.2018.12.025. Epub 2019 Jan 23.
Animal and human studies show that exposure to solar-simulated UVR is immunomodulatory. Human studies that used natural sun exposure and controlled for confounding are rare. We immunized 217 healthy adults (age range = 18-40 years) with a T-cell-dependent antigen, keyhole limpet hemocyanin, and measured personal clothing-adjusted UVR exposure (for 5 days before and after immunization), lifetime cumulative UVR exposure, serum 25-hydroxyvitamin D concentration at immunization, and potential confounding factors. We tested cellular and humoral immune responses in relation to UVR exposure. The delayed-type hypersensitivity response to keyhole limpet hemocyanin recall challenge was lower in individuals with higher personal clothing-adjusted UVR exposure on the day before immunization (P = 0.015) and during intervals spanning the day before to 2-3 days after immunization. There was an incremental increase in T helper type 17 cells (as a proportion of CD4 T cells) from preimmunization to postimmunization in the high, compared with the low, personal clothing-adjusted UVR exposure group (0.31% vs. -0.39%, P = 0.004). Keyhole limpet hemocyanin-specific antibody titers were not associated with acute or cumulative UVR exposure or serum 25-hydroxyvitamin D levels. Higher UVR exposure at antigen sensitization was associated with a reduced delayed-type hypersensitivity response and altered T helper type 17 kinetics. This has implications for the effectiveness of vaccinations and susceptibility to infections that rely on cell-mediated immune responses.
动物和人体研究表明，暴露于模拟太阳紫外线辐射会对免疫系统产生调节作用。使用自然阳光暴露并控制混杂因素的人体研究很少。我们用一种 T 细胞依赖性抗原（血蓝蛋白）对 217 名健康成年人（年龄范围为 18-40 岁）进行了免疫接种，并测量了个人穿着调整后的紫外线辐射暴露量（免疫接种前 5 天和后 5 天）、终生累积紫外线辐射暴露量、免疫接种时的血清 25-羟维生素 D 浓度以及潜在的混杂因素。我们检测了细胞和体液免疫反应与紫外线辐射暴露的关系。在免疫接种前一天和免疫接种前一天至 2-3 天期间，个人穿着调整后的紫外线辐射暴露量较高的个体对血蓝蛋白回忆性挑战的迟发型超敏反应较低（P = 0.015）。与低个人穿着调整紫外线辐射暴露组相比，高个人穿着调整紫外线辐射暴露组在预免疫至免疫后期间，辅助性 T 细胞 17 型（作为 CD4 T 细胞的比例）呈递增增加（0.31%比-0.39%，P = 0.004）。血蓝蛋白特异性抗体滴度与急性或累积紫外线辐射暴露或血清 25-羟维生素 D 水平无关。在抗原致敏时接受更高的紫外线辐射暴露与较低的迟发型超敏反应和辅助性 T 细胞 17 型动力学改变有关。这对依赖细胞介导免疫反应的疫苗接种效果和感染易感性有影响。
