Wang Zhen, Solanki Manoj Kumar, Yu Zhuo-Xin, Yang Li-Tao, An Qian-Li, Dong Deng-Feng, Li Yang-Rui
Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, China.
Key Laboratory of Sugarcane Biotechnology and Genetic Improvement Guangxi, Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning, China.
Front Microbiol. 2019 Jan 9;9:3262. doi: 10.3389/fmicb.2018.03262. eCollection 2018.
Drought directly affects sugarcane production. Plant growth-promoting bacteria have gained attention as growth promoters of plants under abiotic stresses. The present study focused on genome assessment of the plant-beneficial endophyte WZS021 and its vital role in sugarcane plants under drought stress. Based on plant growth-promoting trait analyses, WZS021 had multiple abilities, including tolerance to drought and production of 1-aminocyclopropane-1-carboxylic deaminase, siderophores, and indole acetic acid. We confirmed root colonization of sugarcane transplants by WZS021 by a sterile sand assay and scanning electron microscopy. Plants inoculated with strain WZS021 had a positive influence on the root parameters such as length and biomass when compared to the control plants. A comparative study of the responses of two sugarcane varieties (ROC22 and B8) to different levels of drought stress in the presence or absence of WZS021 was conducted by assessing the plant chemistry. The expression of antioxidants in sugarcane leaves varied with water stress level. WZS021 inoculation improved the contents of chlorophyll, proline, and phytohormones, revealing some potential for the mechanisms by which this strain improves drought tolerance in sugarcane plants. We identified several genes that might be involved in the plant growth- and drought tolerance-promoting effects of this strain.
干旱直接影响甘蔗产量。植物促生细菌作为非生物胁迫下植物的生长促进剂受到了关注。本研究聚焦于植物有益内生菌WZS021的基因组评估及其在干旱胁迫下对甘蔗植株的重要作用。基于植物促生性状分析，WZS021具有多种能力，包括耐旱性以及产生1-氨基环丙烷-1-羧酸脱氨酶、铁载体和吲哚乙酸的能力。我们通过无菌沙培试验和扫描电子显微镜证实了WZS021对甘蔗移栽苗根部的定殖。与对照植株相比，接种菌株WZS021的植株对根参数如长度和生物量有积极影响。通过评估植物化学性质，对两个甘蔗品种（ROC22和B8）在有或无WZS021存在的情况下对不同程度干旱胁迫的响应进行了比较研究。甘蔗叶片中抗氧化剂的表达随水分胁迫水平而变化。接种WZS021提高了叶绿素、脯氨酸和植物激素的含量，揭示了该菌株提高甘蔗植株耐旱性机制的一些潜力。我们鉴定了几个可能参与该菌株促进植物生长和耐旱作用的基因。
