Nelson Clarke, Khan Yusuf, Laurencin Cato T
The Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, Institute for Regenerative Engineering, University of Connecticut Health Center, School of Medicine, Farmington, Connecticut 06030.
Department of Orthopaedic Surgery, University of Connecticut Health Center, School of Medicine, Farmington, Connecticut 06030.
Regen Eng Transl Med. 2018 Sep;4(3):133-141. doi: 10.1007/s40883-018-0055-1. Epub 2018 Jun 14.
The demand for bone grafts has led to advances in regenerative engineering, a field at the intersection of advanced biomaterials, stem cell science, physics, developmental biology, and clinical translation. In this work, the authors evaluated a hybrid nanofiber/microsphere matrices both and for its ability to promote bone regeneration. Quantitative measures of cellular characteristics showed a higher fraction of marrow stromal cells with collagen promoter activity on hybrid matrices compared to control matrices (41% vs. 24%, p = 0.02). Control and hybrid matrices were then implanted for 6 weeks in calvarial defects of mice, and the animals received a single injection of calcein 1 day prior to sacrifice to visualize bone formation. Cryohistology of the undecalfied implants were evaluated for markers of bone mineralization, which revealed evidence of higher levels of bone tissue formation in hybrid matrices compared to controls. These data provide support that nanofiber-permeated, sintered, composite microsphere matrices may be a particularly useful matrix for the regenerative engineering of bone.
对骨移植的需求推动了再生工程领域的发展，该领域处于先进生物材料、干细胞科学、物理学、发育生物学和临床转化的交叉点。在这项工作中，作者评估了一种混合纳米纤维/微球基质在促进骨再生方面的能力。细胞特征的定量测量结果显示，与对照基质相比，混合基质上具有胶原蛋白启动子活性的骨髓基质细胞比例更高（41% 对 24%，p = 0.02）。然后将对照和混合基质植入小鼠颅骨缺损处6周，在处死前1天给动物单次注射钙黄绿素以观察骨形成情况。对未脱钙植入物进行冷冻组织学评估，以检测骨矿化标记物，结果显示与对照相比，混合基质中骨组织形成水平更高。这些数据支持了纳米纤维渗透、烧结的复合微球基质可能是骨再生工程中特别有用的基质这一观点。
