Takahashi K, Maita K, Shirasu Y, Taniguchi H, Yoshikawa Y
Toxicology Division, Institute of Environmental Toxicology, Kodaira-shi, Japan.
Vet Pathol. 1988 Sep;25(5):337-42. doi: 10.1177/030098588802500501.
Anti-rat myoglobin (Mb) was prepared and used in the avidin-biotin-peroxidase complex (ABC) method on paraffin-embedded sections of nine soft tissue tumors (including two rhabdomyosarcomas) of rats. Distribution and nature of the reactive substance to Mb antiserum were compared to those of desmin antiserum. Rat Mb was isolated from the skeletal muscle; monospecificity of the rat antiserum was confirmed by the immunoblotting procedures. The Mb antiserum reacted specifically to normal and neoplastic striated muscle cells. Mb-staining reactions were present diffusely in the cytoplasm, while desmin-staining substances were localized at Z-bands or were diffuse in the cytoplasm as separated aggregates. Reaction to the Mb serum was also detected in cells of thick portions of Henle's loop and distal convoluted tubules.
制备了抗大鼠肌红蛋白（Mb），并将其用于抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，对9只大鼠软组织肿瘤（包括2例横纹肌肉瘤）的石蜡包埋切片进行检测。将对Mb抗血清的反应性物质的分布和性质与结蛋白抗血清的进行比较。大鼠Mb从骨骼肌中分离出来；通过免疫印迹法证实了大鼠抗血清的单特异性。Mb抗血清与正常和肿瘤性横纹肌细胞发生特异性反应。Mb染色反应弥漫存在于细胞质中，而结蛋白染色物质定位于Z带或作为分离的聚集体弥漫于细胞质中。在髓袢厚段和远曲小管的细胞中也检测到了对Mb血清的反应。
