Corson J M, Pinkus G S
Am J Pathol. 1981 Jun;103(3):384-9.
Intracellular myoglobin represents an excellent marker for specific characterization of normal (adult and fetal) and malignant skeletal muscle cells in paraffin sections. With an immunoperoxidase indirect sandwich technique for detection of intracellular myoglobin, positive staining was observed in 13 of 17 rhabdomyosarcoma specimens including 5 of 7 of the alveolar type, 5 of 5 of the embryonal type, and 3 of 5 of the pleomorphic type. Initial fixation in Zenker's-acetic acid solution gave optimal staining, but satisfactory results were obtained with fixation in formalin, Bouin's, and B5 solutions. Other types of sarcomas (13 cases) and other types of tumors (24 cases) that sometimes mimic rhabdomyosarcoma on histologic examination gave negative results. The immunoperoxidase method affords a sensitive and specific method for identifying rhabdomyoblasts in tissue on the basis of intracellular myoglobin and is of use in distinguishing rhabdomyosarcomas from other sarcomas and from malignant tumors of other types.
细胞内肌红蛋白是石蜡切片中正常（成人和胎儿）及恶性骨骼肌细胞特异性鉴定的优良标志物。采用免疫过氧化物酶间接夹心技术检测细胞内肌红蛋白，在17例横纹肌肉瘤标本中的13例观察到阳性染色，其中包括7例肺泡型中的5例、5例胚胎型中的5例以及5例多形型中的3例。最初用岑克尔氏乙酸溶液固定可获得最佳染色效果，但用福尔马林、布安氏液和B5溶液固定也能得到满意结果。其他类型的肉瘤（13例）和其他类型的肿瘤（24例）在组织学检查时有时会与横纹肌肉瘤相似，但结果均为阴性。免疫过氧化物酶法为基于细胞内肌红蛋白鉴定组织中的横纹肌母细胞提供了一种灵敏且特异的方法，有助于将横纹肌肉瘤与其他肉瘤及其他类型的恶性肿瘤区分开来。
