Department of Organic and Inorganic Chemistry, University of Oviedo, Avenida Julián Clavería 8, 33006, Oviedo, Spain.
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands.
J Biotechnol. 2019 Mar 10;293:24-35. doi: 10.1016/j.jbiotec.2018.12.018. Epub 2019 Jan 26.
Deep eutectic solvents (DES) are a class of neoteric solvents used in multiple applications amongst which biocatalytic processes. Due to its simple preparation, low cost and inherent biodegradable properties, its use as a non-volatile biocompatible co-solvent with both whole cells and isolated enzymes has displayed increased enzyme activity and stability translating to higher product conversions and a surprising higher enantioselectivity in a range of biotransformations. This review lays out the latest updates on the use of DES in redox biocatalytic reactions. With that purpose, a clear division has been made to summarize the application of DES in bioreduction reactions using whole cells and purified alcohol dehydrogenases, oxidations involving alcohol dehydrogenases, heme-dependent enzymes, peroxidases, laccases and catalases, ozonolysis reactions, and finally mention the application of lipase in the mediation of chemoenzymatic epoxidation and Baeyer-Villiger reactions.
深共熔溶剂 (DES) 是一类新型溶剂，在多种应用中都有使用，包括生物催化过程。由于其制备简单、成本低且固有可生物降解特性，因此将其用作具有非挥发性和生物相容性的共溶剂，无论是用于完整细胞还是分离的酶，都显示出提高了酶的活性和稳定性，从而在多种生物转化中实现了更高的产物转化率和令人惊讶的更高对映选择性。本综述概述了深共熔溶剂在氧化还原生物催化反应中的最新应用。为此，我们进行了明确的划分，以总结使用深共熔溶剂在使用完整细胞和纯化醇脱氢酶的生物还原反应、涉及醇脱氢酶、血红素依赖性酶、过氧化物酶、漆酶和过氧化氢酶的氧化反应、臭氧分解反应中的应用，最后提到脂肪酶在介导化学酶促环氧化和 Baeyer-Villiger 反应中的应用。
