College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
Plant Physiol Biochem. 2019 Mar;136:204-214. doi: 10.1016/j.plaphy.2019.01.017. Epub 2019 Jan 17.
Magnesium (Mg) is an essential element for plant growth. Its transport and homeostasis in plants is mainly maintained by the MRS2/MGT of Mg transporters. Little is known about the MRS2/MGT gene family in Brassica napus L. (B. napus), one of the most important oil grains. In our present study, we identified 36 putative MRS2/MGT genes (BnMGTs) from B. napus and investigated their phylogeny, expression pattern and function. These BnMGT genes were sorted into five distinguished groups by the phylogenetic analysis, and they were clearly homologous with the MRS2/MGT genes in Arabidopsis and rice. Complementation assays using the Salmonella typhimurium mutant MM281 demonstrated that the BnMGT genes were capable of mediating Mg uptake and transport, with varied affinities to Mg. The expression pattern analysis showed that the expression of BnMGTs were tissue-specific and varied in different tissues. This work provides the molecular basis to discover the function of BnMGT gene family in plant growth and development.
镁（Mg）是植物生长的必需元素。植物中镁的运输和内稳态主要由镁转运蛋白的 MRS2/MGT 维持。关于油菜（B. napus）中 MRS2/MGT 基因家族的了解甚少，油菜是最重要的油籽之一。在本研究中，我们从油菜中鉴定了 36 个假定的 MRS2/MGT 基因（BnMGTs），并研究了它们的系统发育、表达模式和功能。这些 BnMGT 基因通过系统发育分析分为五个不同的组，它们与拟南芥和水稻的 MRS2/MGT 基因明显同源。使用鼠伤寒沙门氏菌突变体 MM281 的互补测定表明，BnMGT 基因能够介导镁的摄取和运输，对镁的亲和力不同。表达模式分析表明，BnMGTs 的表达具有组织特异性，在不同组织中存在差异。这项工作为发现 BnMGT 基因家族在植物生长发育中的功能提供了分子基础。
