New insights into comprehensive analysis of magnesium transporter () gene family in rice ( L.).

UNLABELLED

Magnesium transporters (MGTs) regulate magnesium absorption, transport, and redistribution in higher plants. To investigate the role of the gene family members under salt stress, this study analyzed the protein properties, gene structure, phylogenetic relationship, synteny patterns, expression, and co-expression networks of 23 non-redundant . The evolutionary relationship of the OsMGT gene family was fully consistent with their functional domain, and were divided into three main classes based on the conserved domain: MMgT, CorA-like, and NIPA. The α/β patterns in the protein structures were highly similar in the CorA-like and NIPA members, with the conserved structures in the Mg-binding and catalytic regions. The CorA-like clade-related proteins demonstrated the highest numbers of protein channels with Pro, Ser, Lys, Gly, and Tyr, as the critical binding residues. The expression analysis of genes in various tissues showed that ' gene family may possess critical functions during rice development. Gene expression analysis of candidate using reverse-transcription quantitative real-time PCR (RT-qPCR) found that four genes exhibited different expression patterns in salt-sensitive and salt-tolerant rice genotypes. We hypothesize that the gene family members may be involved in responses to salt stress. These findings could be useful for further functional investigation of as well as defining their involvement in abiotic stress studies.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-023-03735-4.