白藜芦醇可缓解脂多糖诱导的 PC-12 细胞和大鼠模型的炎症反应。
Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model.
机构信息
Department of Spinal Surgery, Dalian Municipal Central Hospital, No.826, Xinan Road, Shahekou District, Dalian, 116033, Liaoning, China.
出版信息
BMC Biotechnol. 2019 Jan 28;19(1):10. doi: 10.1186/s12896-019-0502-1.
BACKGROUND
Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS.
RESULTS
RSV improved inflammatory injury and functional recovery in rat model of SCI. RSV inhibited LPS-induced inflammatory injury in PC-12 cells via increasing viability, decreasing apoptosis, and suppressing IL-1β, IL-6, and TNF-α expression. miR-132 was down-regulated after LPS treatment but up-regulated after RSV administration. miR-132 silence curbed the protective effect of RSV. The results including increase of cell growth, suppression of inflammatory response, and blocking of NF-κB and p38MAPK pathways produced by RSV were all reversed by miR-132 silence.
CONCLUSION
RSV could up-regulate miR-132 and further ameliorate inflammatory response in PC-12 cells by inhibiting NF-κB and p38MAPK pathways.
背景
脊髓损伤（SCI）至今仍是一个巨大的医学难题，因为没有医院提供用于修复中枢神经系统和恢复功能的多种策略。在此，我们专注于 PC-12 细胞作为一个重要的研究工具，并研究白藜芦醇（RSV）在 LPS 诱导的炎症中的潜在作用。
结果
RSV 改善了 SCI 大鼠模型中的炎症损伤和功能恢复。RSV 通过增加细胞活力、减少细胞凋亡，并抑制 IL-1β、IL-6 和 TNF-α 的表达，抑制 LPS 诱导的 PC-12 细胞炎症损伤。LPS 处理后 miR-132 下调，但 RSV 给药后上调。miR-132 沉默抑制 RSV 的保护作用。RSV 产生的增加细胞生长、抑制炎症反应以及阻断 NF-κB 和 p38MAPK 通路的作用，均因 miR-132 沉默而逆转。
结论
RSV 可上调 miR-132，并通过抑制 NF-κB 和 p38MAPK 通路进一步改善 PC-12 细胞的炎症反应。
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