酶联免疫吸附测定法与快速血浆反应素在献血者梅毒筛查中的比较评估
Comparative evaluation of enzyme-linked immunosorbent assay with rapid plasma reagin for screening of syphilis in blood donors.
作者信息
Sachdev Suchet, Sharma Arun Kumar, Sethi Sunil, Garg Sachin, Lamba Divjot Singh, Sharma Ratti Ram, Marwaha Neelam
机构信息
Department of Transfusion Medicine, PGIMER, Chandigarh, India.
Department of Gastroenterology, PGIMER, Chandigarh, India.
出版信息
Asian J Transfus Sci. 2018 Jul-Dec;12(2):165-168. doi: 10.4103/ajts.AJTS_126_17.
World Health Organization (WHO) recommends screening of syphilis in low prevalence populations of blood donors by treponemal tests like enzyme-linked immunosorbent assay (ELISA), whereas in India screening is done by rapid plasma reagin (RPR). The present pilot study evaluated the performance of ELISA compared to RPR, keeping hemagglutination assay as a reference test. ELISA was equally sensitive (100%), more specific (56.3% vs. 0%), more accurate (83.7% vs. 62.7%), had better positive predictive value (79.4% vs. 62.8%) and negative predictive value (100% vs. 0%), and less biological false positivity (37.2% vs. 20.6%) when compared to RPR. The WHO recommendations of screening for syphilis in low prevalence population of blood donors using ELISA may be adopted for usage in transfusion services that have the facility of ELISA.
世界卫生组织（WHO）建议通过酶联免疫吸附测定（ELISA）等梅毒螺旋体检测方法，对梅毒低流行率的献血人群进行筛查，而在印度，筛查采用快速血浆反应素环状卡片试验（RPR）。本试点研究以血凝试验作为参考检测方法，评估了ELISA与RPR相比的性能。与RPR相比，ELISA具有同等的敏感性（100%）、更高的特异性（56.3%对0%）、更高的准确性（83.7%对62.7%）、更好的阳性预测值（79.4%对62.8%）和阴性预测值（100%对0%），以及更低的生物学假阳性率（37.2%对20.6%）。对于具备ELISA检测设施的输血服务机构，可采用WHO关于使用ELISA对梅毒低流行率献血人群进行筛查的建议。
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