Medical Science & Research, Deutsche Homöopathie-Union, Karlsruhe, Germany.
AMT e. V., Breitscheid, Germany.
Z Gastroenterol. 2021 May;59(5):423-437. doi: 10.1055/a-1404-3344. Epub 2021 May 12.
is used in traditional West African medicine and in homeopathy for treatment and prevention of several gastrointestinal problems. The aim of this study was to evaluate the effect of repeated doses of two products (10 % ethanolic tincture, mother tincture (MT); 3 decimal potency, 3X) on the microbial activity of physiological human colon microbiota using a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and to investigate any preventive effect against infections with diarrhea-causing pathogens.
Upon inoculation with fecal microbiota from a healthy donor, 4 parallel proximal colon compartments of the SHIME were treated either with MT, 3X, ethanol control or blank control for 7 days. Using the -adapted microbial community from SHIME, 48 h challenge tests were performed with enterotoxigenic (ETEC) and in 4 different doses (10-10 colony forming units as typical infectious doses). Pathogen concentrations, short-chain fatty acids (SCFAs) and branched SCFA production were measured in triplicate at 0, 24 and 48 h.
In the challenge tests, both products were able to restrict the colonization of ETEC and at 3 of the 4 pathogen doses (except the highest doses), with a stronger anti-pathogenic effect for MT, which included a reduction of 2.0 log-units of ETEC (p < 0.0001) and 1.1 log-units of (p < 0.0001). Total SCFA levels remained unaffected, but butyrate increased during the first 24 h (p < 0.0001 for ETEC), accompanied by decreased acetate production.
We observed a systemic activating effect of on intestinal microbiome resistance, which resulted in an anti-pathogenic effect, especially against ETEC. We hypothesize that the mode of action is also based on systemic regulative effects.
在传统的西非医学和顺势疗法中，[药物名称]被用于治疗和预防多种胃肠道问题。本研究的目的是使用人类肠道微生物模拟系统（SHIME）评估两种产品（10%乙醇酊剂、母酊剂（MT）；3 十进制强度，3X）重复剂量对生理人类结肠微生物群的微生物活性的影响，并研究其对腹泻病原体感染的任何预防作用。
在接种来自健康供体的粪便微生物群后，SHIME 的 4 个平行近端结肠隔室分别用 MT、3X、乙醇对照或空白对照处理 7 天。使用来自 SHIME 的 -适应微生物群落，用 48 小时挑战试验用产肠毒素性（ETEC）和 进行，剂量为 4 种不同剂量（典型感染剂量为 10-10 菌落形成单位）。在 0、24 和 48 小时时，以三重重复测量测定病原体浓度、短链脂肪酸（SCFA）和支链 SCFA 产生。
在挑战试验中，两种产品均能够限制 ETEC 和 的定植，在 4 种病原体剂量中的 3 种（除了最高剂量），MT 的抗病原体作用更强，包括 ETEC 减少 2.0 对数单位（p<0.0001）和 减少 1.1 对数单位（p<0.0001）。总 SCFA 水平保持不变，但丁酸盐在最初 24 小时内增加（ETEC 时 p<0.0001），同时乙酸盐的产生减少。
我们观察到 [药物名称]对肠道微生物组抗性的全身性激活作用，这导致了抗病原体作用，特别是针对 ETEC。我们假设其作用方式也基于全身性调节作用。
