Mizuno T, Itoh K
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Mol Gen Genet. 1988 Oct;214(2):249-56. doi: 10.1007/BF00337718.
Until recently it was assumed that any short segment of DNA could be approximated as a straight rod. Many instances, however, have been reported in which the helical axis is curved. We have devised a simple method for selective identification of DNA segments containing a sequence-directed bend (curvature), by means of a two-dimensional polyacrylamide gel electrophoresis. In order to gain general insights into the structural features and the functional significance of sequence-directed bends, a bank of plasmids carrying bent DNA inserts from the Saccharomyces cerevisiae total genomic DNA was constructed. Primary characterizations of a set of bent DNA segments randomly cloned from S. cerevisiae are presented. One of the cloned DNA segments appears to be derived from a yeast plasmid, the 2 microns circle DNA.
直到最近，人们一直认为任何短片段的DNA都可以近似看作是一根直杆。然而，已经有许多报道称，其螺旋轴是弯曲的。我们设计了一种简单的方法，通过二维聚丙烯酰胺凝胶电泳来选择性鉴定含有序列导向弯曲（曲率）的DNA片段。为了全面了解序列导向弯曲的结构特征和功能意义，构建了一组携带来自酿酒酵母全基因组DNA的弯曲DNA插入片段的质粒库。本文展示了从酿酒酵母中随机克隆的一组弯曲DNA片段的初步特征。其中一个克隆的DNA片段似乎源自酵母质粒，即2微米环状DNA。
