MicroSynbiotiX Ltd, 11011 N Torrey Pines Rd Ste. #135, La Jolla, CA, 92037, USA.
MicroSynbiotiX Ltd, 11011 N Torrey Pines Rd Ste. #135, La Jolla, CA, 92037, USA.
Fish Shellfish Immunol. 2019 Apr;87:414-420. doi: 10.1016/j.fsi.2019.01.038. Epub 2019 Jan 28.
Recombinant proteins produced by biological systems such as bacteria, yeasts, mammalian and insect cell cultures are widely used for clinical or industrial purposes. Most therapeutic protein drugs require purification, cold chain, and injection, which make them prohibitively expensive and hinders their widespread use. Here, we describe a new economical oral vaccination platform using algae and evaluated its potential for the delivery of recombinant drugs using GFP expressed in the chloroplast of algal cells. The transplastomic algae expressing recombinant GFPs were freeze-dried for long-term storage at ambient temperature and for easy handling in feeding. GFPs bioencapsulated by lyophilized Chlamydomonas reinhardtii were found intact without degradation for several months at ambient temperature. The expression level of GFP in the lyophilized algae was estimated at 0.47 μg/mg dry weight. The GFPs bioencapsulated and orally delivered to Danio rerio were immunostained and observed in the intestinal tissues using a confocal microscope. Furthermore, the uptaken GFPs in the intestine were detected in the blood using ELISA and the detected level was 5.4 ng of GFP/μl of serum. These results demonstrate that microalgae can be a viable protein production and oral delivery system to vaccinate fish. The results give greater justification to continue exploring the concept of microalgal-based oral vaccines. The potential of the technology would greatly benefit aquaculture farmers by providing them with affordable, environmentally sustainable, and user-friendly vaccines.
通过细菌、酵母、哺乳动物和昆虫细胞培养等生物系统生产的重组蛋白，广泛用于临床或工业用途。大多数治疗性蛋白药物需要纯化、冷链和注射，这使得它们价格过高，阻碍了它们的广泛应用。在这里，我们描述了一种使用藻类的新的经济口服疫苗接种平台，并评估了其在通过藻类细胞叶绿体中表达的 GFP 递呈重组药物方面的潜力。表达重组 GFP 的转基因藻类被冻干，以便在室温下长期储存，并便于在喂养时处理。在室温下，几个月内 GFP 生物包封在冻干的莱茵衣藻中没有降解，保持完整。冻干藻类中 GFP 的表达水平估计为 0.47 μg/mg 干重。GFP 被生物包封并口服给予斑马鱼，使用共聚焦显微镜在肠组织中进行免疫染色和观察。此外，通过 ELISA 在血液中检测到肠内吸收的 GFP，检测水平为 5.4 ng GFP/μl 血清。这些结果表明，微藻可以成为一种可行的蛋白质生产和口服递呈系统，用于给鱼类接种疫苗。这些结果为继续探索基于微藻的口服疫苗的概念提供了更多的依据。该技术的潜力将通过提供负担得起、环境可持续且用户友好的疫苗，使水产养殖者大大受益。
