固态发酵菌丝体提取物对 2 型糖尿病大鼠的降血糖和降血脂作用。

Hypoglycemic and Hypolipidemic Effects of Mycelial Extract from Solid-State Culture in A Rat Model of Type 2 Diabetes.

机构信息

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Department of Chemistry, University of California, Davis, CA 95616, USA.

出版信息

Nutrients. 2019 Jan 30;11(2):296. doi: 10.3390/nu11020296.

Hypoglycemic and hypolipidemic effects of have been observed in numerous studies, but the underlying molecular mechanisms are unclear. In this study, we prepared extract (PLE) from mycelia of solid-state culture, and evaluated its hypoglycemic and hypolipidemic effects in rat models of high-fat diet (HFD)-induced and low-dose streptozotocin (STZ)-induced type 2 diabetes. PLE treatment effectively reduced blood glucose levels, and improved insulin resistance and lipid and lipoprotein profiles. The hypoglycemic effect of PLE was based on inhibition of key hepatic gluconeogenesis enzymes (FBPase, G6Pase) expression and hepatic glycogen degradation, and consequent reduction of hepatic glucose production. PLE also: (i) enhanced expression of CPT1A and ACOX1 (key proteins involved in fatty acid β-oxidation) and low-density lipoprotein receptor (LDLR) in liver, thus promoting clearance of triglycerides and LDL-C; (ii) inhibited expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) in liver, thus reducing cholesterol production; (iii) displayed strong hepatoprotective and renal protective effects. Our findings indicate that PLE has strong potential functional food application in adjuvant treatment of type 2 diabetes with dyslipidemia.

在许多研究中观察到了[药物名称]的降血糖和降血脂作用，但潜在的分子机制尚不清楚。在这项研究中，我们从固态培养的菌丝体中制备了[药物名称]提取物（PLE），并在高脂肪饮食（HFD）诱导和低剂量链脲佐菌素（STZ）诱导的 2 型糖尿病大鼠模型中评估了其降血糖和降血脂作用。PLE 治疗可有效降低血糖水平，并改善胰岛素抵抗以及血脂和脂蛋白谱。PLE 的降血糖作用基于抑制关键的肝糖异生酶（FBPase、G6Pase）表达和肝糖原降解，从而减少肝葡萄糖生成。PLE 还：（i）增强肝脏中 CPT1A 和 ACOX1（参与脂肪酸β氧化的关键蛋白）和低密度脂蛋白受体（LDLR）的表达，从而促进甘油三酯和 LDL-C 的清除；（ii）抑制肝脏中 3-羟-3-甲基戊二酰辅酶 A 还原酶（HMGCR）的表达，从而减少胆固醇的产生；（iii）显示出很强的肝保护和肾保护作用。我们的研究结果表明，PLE 在辅助治疗伴有血脂异常的 2 型糖尿病方面具有很强的功能性食品应用潜力。