Chen Lixiang, Cao Changli, Wang Shuhua, Varcoe John R, Slade Robert C T, Avignone-Rossa Claudio, Zhao Feng
CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, P.R.China; University of Chinese Academy of Sciences, Beijing 100049, P.R.China.
Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315830, P.R. China.
iScience. 2019 Feb 22;12:260-269. doi: 10.1016/j.isci.2019.01.020. Epub 2019 Jan 21.
Elucidating the effect of harsh environments on the activities of microorganisms is important in revealing how microbes withstand unfavorable conditions or evolve mechanisms to counteract those effects, many of which involve electron transfer phenomena. Here we show that the non-acidophilic and non-thermophilic Bacillus subtilis is able to maintain activity after being subjected to extreme temperatures (100°C for up to 8 h) and acidic environments (pH = 1.50 for over 2 years). In the process, our results suggest that B. subtilis utilizes an extracellular electron transfer as an electron communication pathway between B. subtilis and the environment that involves the cofactor nicotinamide adenine dinucleotide as an essential participant to maintain viability. Elucidation of the capability of the non-acidophilic and non-thermophilic strain to maintain viability under these extreme conditions could aid in understanding the cell responses to different environments from the perspective of energy conservation pathways.
阐明恶劣环境对微生物活性的影响，对于揭示微生物如何抵御不利条件或进化出应对这些影响的机制至关重要，其中许多机制都涉及电子转移现象。在此，我们表明非嗜酸且非嗜热的枯草芽孢杆菌在经受极端温度（100°C长达8小时）和酸性环境（pH = 1.50超过2年）后仍能保持活性。在此过程中，我们的结果表明枯草芽孢杆菌利用细胞外电子转移作为其与环境之间的电子通讯途径，其中辅因子烟酰胺腺嘌呤二核苷酸是维持生存能力的必需参与者。阐明这种非嗜酸且非嗜热菌株在这些极端条件下维持生存能力的机制，有助于从能量守恒途径的角度理解细胞对不同环境的反应。
